| ALX-746-023-C050 | 50 µg | 232.00 USD |  |
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| Alternative Name: | Oligo(dA:dT) |
| Source: | Synthetic. |
| Quantity: | Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 0.01-0.025µg/ml. |
| Formulation: | Lyophilized. Sterile. |
| Endotoxin Content: | <0.0002EU/µg (LAL test; BioWhittaker) |
| Reconstitution: | For a 250µg/ml stock solution, dissolve the total vial content in 200µl endotoxin-free buffer or water. |
| Shipping: | Ambient Temperature |
| Long Term Storage: | +4°C |
| Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
| Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
| Technical Info/Product Notes: | For in vitro stimulation we recommend the following procedure: 1. Bone marrow-derived mouse dendritic cells (BM mDC) are cultured 8x104/100μl in RPMI containing 10% FCS over night at 5% CO2 and 37°C. 2. The medium is removed and replaced by 100μl OPTI MEM (Invitrogen) and subsequently the transfection mixture is added: 25μl OPTI MEM containing 0.025 to 0.25 Poly(dA:dT) + 25μl OPTI MEM containing 0.5μl Lipofectamine 2000 (Invitrogen) or alternative transfection agents. 3. Over night stimulation at 5% CO2 and 37°C. 4. Measurement of IFN-α/β in culture supernatants. |
| Regulatory Status: | RUO - Research Use Only |
Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE®) (synthetic) |
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| Activator of TLR3 and MDA5 | ||||||
| 31852-29-6, Synthetic. | Print as PDF | ||||||
| ALX-746-021-M002 | 2 mg | 157.00 USD | |
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| ALX-746-021-M005 | 5 mg | 208.00 USD | |
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PBS (endotoxin-free) (sterile) |
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| Print as PDF | ||||||
| ALX-505-007-LD15 | 1.5 ml | 67.00 USD | |
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| ALX-505-007-L010 | 10 ml | 132.00 USD | |
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ddWater (endotoxin-free) (sterile) |
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| 7732-18-5 | Print as PDF | ||||||
| ALX-505-008-LD15 | 1.5 ml | 50.00 USD | |
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