For a larger pack size, please see Prod. No.
ALX-746-053.
CpG oligodeoxynucleotide (Type A) optimized for NK cell activation with mixed phosphodiester/phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).
Product Details
Sequence: | 5’-ggGGTCAACGTTGAgggggg-3’(lower case letters indicate phosphorothioate linkage). |
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MW: | 6643 (ammonium salt) |
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Source: | Synthetic. |
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Quantity: | 14.9nmol (~99µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate). |
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Formulation: | Lyophilized. Sterile. |
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Purity Detail: | Activity and endotoxin tested - TLRGRADE®. |
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Endotoxin Content: | <0.002EU/µg (LAL test; BioWhittaker) |
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Reconstitution: | For a 100µM stock solution, dissolve the total vial content in 149µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving. |
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Shipping: | Ambient |
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Long Term Storage: | +4°C |
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Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
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Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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Technical Info/Product Notes: | Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For inactive control compound, please see Prod. No. ALX-746-203. |
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Regulatory Status: | RUO - Research Use Only |
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General Literature References
Induction of protective immunity against MHC class I-deficient, HPV16-associated tumours with peptide and dendritic cell-based vaccines: M. Reinis, et al.; Int. J. Oncol.
36, 545 (2010),
Abstract;
Divergent therapeutic and immunologic effects of oligodeoxynucleotides with distinct CpG motifs: Z.K. Ballas, et al.; J. Immunol.
167, 4878 (2001),
Abstract;
Interleukin-12- and gamma interferon-dependent protection against malaria conferred by CpG oligodeoxynucleotide in mice: R.A. Gramzinski, et al.; Infect. Immun.
69, 1643 (2001),
Abstract;
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