ODN 1668 (TLRGRADE^®) (synthetic)

TLR9 ligand



ALX-746-001-C100	100 µg	187.00 USD
Do you need bulk/larger quantities?

For a larger pack size, please see Prod. No. ALX-746-051.

CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).

Product Details

Sequence:	5’-tccatgacgttcctgatgct-3’ (lower case letters indicate phosphorothioate linkage).

MW:	6383 (ammonium salt)

Source:	Synthetic.

Quantity:	100µg (~15nmol). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).

Formulation:	Lyophilized. Sterile.

Purity Detail:	Activity and endotoxin tested - TLRGRADE^®.

Endotoxin Content:	<0.002EU/µg (LAL test; BioWhittaker)

Reconstitution:	For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.

Shipping:	Ambient Temperature

Long Term Storage:	+4°C

Use/Stability:	Aqueous stock solution is stable for 1 day when stored at +4°C.

Handling:	Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.

Technical Info/Product Notes:	Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For inactive control compound, please see Prod. No. ALX-746-200.

Regulatory Status:	RUO - Research Use Only

ODN 1720 (TLRGRADE) (synthetic) (Control) image

Figure 1: TLR9-dependent activation of mouse macrophages by ODN 1668 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-001). Method: Culture of 5x10⁵ cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell activation was measured by IL12-p40 induction. Only CpG ODN 1668 (Prod. No. ALX-746-001), but not the corresponding GpC control ODN 1720 (Prod. No. ALX-746-200) activates wild type mouse macrophages. Activation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5µg/ml).

Figure 2: TLR9-dependent mitogenic activity of ODN 1668 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-001) in mouse splenocytes. Method: Culture of 5x10⁵ cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell proliferation was analyzed after 72 hours with a 16 hours³H-thymidine pulse.Only CpG ODN 1668 (Prod. No. ALX-746-001), but not the corresponding GpC control ODN 1720 (Prod. No. ALX-746-200) induces proliferation of wild type mouse splenocytes. Induction of cell proliferation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5µg/ml).

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Product Literature References

Distinct effects of different adjuvants in the mouse model of allergic airway inflammation: H.S. Shin, et al.; Asian Pac. J. Allergy Immunol. 40, 111 (2022), Abstract;

Paneth Cells Regulate Lymphangiogenesis under Control of Microbial Signals during Experimental Portal Hypertension: M. Hassan, et al.; Biomedicines 10, 1503 (2022), Abstract;

Ly6C+ monocyte efferocytosis and cross-presentation of cell-associated antigens: S.R. Larson, et al.; Cell Death Differ. 23, 997 (2016), Application(s): Efferocytosis assay, in vivo studies, Abstract; Full Text

Activation of murine macrophages via TLR2 and TLR4 is negatively regulated by a Lyn/PI3K module and promoted by SHIP1: S. Keck, et al.; J. Immunol. 184, 5809 (2010), Abstract; Full Text

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