Causes massive neurotransmitter release from a wide variety of central and peripheral synaptic junctions of vertebrates using Ca2+-dependent and Ca2+-independent pathways. A useful pharmacological tool in the studies of synaptic vesicles exocytosis of different neutrotransmitters.
Product Details
| MW: | ~130kDa. |
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| Source: | Isolated from Latrodectus tredecimguttatus. |
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| CAS: | 65988-34-3 |
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| RTECS: | OE9020000 |
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| UniProt ID: | P23631 |
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| Quantity: | Determined by Lowry and Pierce method. |
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| Formulation: | Lyophilized. |
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| Purity: | ≥97% |
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| Purity Detail: | Migrates as one distinct band when run on a 5-25% gradient polyacrylamide gel according to the method of Laemmli (Nature 227, 680 (1970)). |
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| Quality Control: | Bioassay: Test of the ability to stimulate neurotransmitter release in both Ca2+-free and Ca2+-containing media according to Valtorta. Effective concentration: 100pM-1nM. |
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| Reconstitution: | Reconstitute in 0.5ml of distilled cold water. Stir very gently. Do not shake or vortex. Add 0.5 ml glycerol. Stock solution received contains 50% glycerol and 300nM α-latrotoxin. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solution is stable for 1 week when stored at +4°C or for at least one year at -20°C. |
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| Technical Info/Product Notes: | Attention: For best results, only proceed according to the given instructions. Dissolution and/or storage of this toxin contrary to these instructions may affect the stability and biological activity of this compound. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Activity-dependent regulation of mitochondrial motility in developing cortical dendrites: C.A. Silva, et al.; Elife
7554, e62901 (2021),
Abstract;
Cell-Based Reporter Release Assay to Determine the Activity of Calcium-Dependent Neurotoxins and Neuroactive Pharmaceuticals: A.P. Neuschäfer-Rube, et al.; Toxins (Basel)
13, 247 (2021),
Abstract;
Full Text
Neurexin-1α regulates neurite growth of rat hippocampal neurons: A. Wang, et al.; Int. J. Physiol. Pathophysiol. Pharmacol.
11, 115 (2019),
Abstract;
Full Text
Alpha-latrotoxin induces exocytosis by inhibition of voltage-dependent K+ channels and by stimulation of L-type Ca2+ channels via latrophilin in beta-cells: S. Lajus, et al.; J. Biol. Chem.
281, 5522 (2006),
Abstract;
alpha-Latrotoxin and its receptors: neurexins and CIRL/latrophilins: T.C. Sudhof; Annu. Rev. Neurosci.
24, 933 (2001), (Review),
Abstract;
Mechanisms of alpha-latrotoxin action: A. W. Henkel & S. Sankaranarayanan; Cell Tissue Res.
296, 229 (1999), (Review),
Abstract;
Alpha-latrotoxin stimulates glutamate release from cortical astrocytes in cell culture: V. Parpura, et al.; FEBS Lett.
360, 266 (1995),
Abstract;
GTP cleavage by the small GTP-binding protein Rab3A is associated with exocytosis of synaptic vesicles induced by alpha-latrotoxin: B. Stahl, et al.; J. Biol. Chem.
269, 24770 (1994),
Abstract;
Full Text
The effect of alpha-latrotoxin on a synaptic connection between identified neurons in the brain of the mollusc Helix pomatia L: O.N. Osipenko, et al.; Toxicon
31, 1123 (1993),
Abstract;
Synaptophysin (p38) at the frog neuromuscular junction: its incorporation into the axolemma and recycling after intense quantal secretion: F. Valtorta, et al.; J. Cell. Biol.
107, 2717 (1988),
Abstract;
Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus): A. Grasso; Biochim. Biophys. Acta
439, 406 (1976),
Abstract;
Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions: N. Frontali, et al.; J. Cell. Biol.
68, 462 (1976),
Abstract;
