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DAF-FM DA (cell permeable)

Fluorescent probe for NO detection. Cell permeable.
ALX-620-071-M001 1 mg 883.00 USD
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Cell permeable photo-stable nitric oxide (NO) fluorescent indicator, that is hydrolyzed to DAF-FM by intracellular esterases (Ex: 500nm; Em: 515nm). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Not for sale in Japan.

Product Details

Alternative Name:Diaminofluorescein-FM . diacetate
Formulation:Dissolved in 0.4ml DMSO.
Purity:≥98% (HPLC)
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
Handling:Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins: F. Vargas-Romero, et al.; Microb. Pathog. 100, 305 (2016), Application(s): Nitric oxide determination, bovine, Abstract;
Limits in the use of cPTIO as nitric oxide scavenger and EPR probe in plant cells and seedlings: S. D'Alessandro, et al.; Front. Plant Sci. 4, 340 (2013), Application(s): Confocal microscopic analysis of NO production in plant cells, Abstract; Full Text
TNF-α and IFN-γ are potential inducers of Fas-mediated keratinocyte apoptosis through activation of inducible nitric oxide synthase in toxic epidermal necrolysis: I. Viard-Leveugle, et al.; J. Invest. Dermatol. 133, 489 (2013), Application(s): Flow cytometry analysis of NO production in mammalian cells, Abstract;
Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe: S. Lepiller et al.; Free Radic. Biol. Med. 43, 619 (2007), Abstract;
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001), Abstract;
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem. 76, 1404 (2001), Abstract;
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem. 287, 203 (2000), Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),

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