Cell permeable photo-stable nitric oxide (NO) fluorescent indicator, that is hydrolyzed to DAF-FM by intracellular esterases (Ex: 500nm; Em: 515nm). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Not for sale in Japan.
Product Details
| Alternative Name: | Diaminofluorescein-FM . diacetate |
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| Formula: | C25H18F2N2O7 |
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| MW: | 496.4 |
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| CAS: | 254109-22-3 |
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| Formulation: | Dissolved in 0.4ml DMSO. |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Liquid. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions. |
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| Handling: | Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins: F. Vargas-Romero, et al.; Microb. Pathog.
100, 305 (2016),
Application(s): Nitric oxide determination, bovine,
Abstract;
Limits in the use of cPTIO as nitric oxide scavenger and EPR probe in plant cells and seedlings: S. D'Alessandro, et al.; Front. Plant Sci.
4, 340 (2013),
Application(s): Confocal microscopic analysis of NO production in plant cells,
Abstract;
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TNF-α and IFN-γ are potential inducers of Fas-mediated keratinocyte apoptosis through activation of inducible nitric oxide synthase in toxic epidermal necrolysis: I. Viard-Leveugle, et al.; J. Invest. Dermatol.
133, 489 (2013),
Application(s): Flow cytometry analysis of NO production in mammalian cells,
Abstract;
Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe: S. Lepiller et al.; Free Radic. Biol. Med.
43, 619 (2007),
Abstract;
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem.
73, 1967 (2001),
Abstract;
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem.
76, 1404 (2001),
Abstract;
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem.
287, 203 (2000),
Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),
