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DAR-4M AM (cell permeable)

Fluorescent probe for NO detection. Cell permeable.
ALX-620-069-M001 1 mg 845.00 USD
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Cell permeable analog of DAR-4M (Prod. No. ALX-620-067). Can be used to detect nitric oxide (NO) directly in cell specimens prepared from various sources, such as the brain (Ex: ~560nm; Em: 575nm). Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM. Not for sale in Japan.

Product Details

Alternative Name:Diaminorhodamine-4M AM
Formula:C28H31N4O5 . I
MW:503.6 . 126.9
Formulation:Dissolved in 0.32ml DMSO.
Purity:≥98% (HPLC)
Appearance:Reddish-violet liquid.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
Handling:Warm to room temperature before opening. Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Endothelial CaMKII as a regulator of eNOS activity and NO-mediated vasoreactivity: S. Murthy, et al.; PLoS One 12, e0186311 (2017), Abstract; Full Text
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001), Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & N. Nagano ; Advanced Materials 12, 763 (2000),

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