Cell permeable analog of DAR-4M (Prod. No. ALX-620-067). Can be used to detect nitric oxide (NO) directly in cell specimens prepared from various sources, such as the brain (Ex: ~560nm; Em: 575nm). Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM. Not for sale in Japan.
Product Details
| Alternative Name: | Diaminorhodamine-4M AM |
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| Formula: | C28H31N4O5 . I |
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| MW: | 503.6 . 126.9 |
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| Concentration: | ~5mM |
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| Formulation: | Dissolved in 0.32ml DMSO. |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Reddish-violet liquid. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions. |
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| Handling: | Warm to room temperature before opening. Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Endothelial CaMKII as a regulator of eNOS activity and NO-mediated vasoreactivity: S. Murthy, et al.; PLoS One
12, e0186311 (2017),
Abstract;
Full Text
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem.
73, 1967 (2001),
Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & N. Nagano ; Advanced Materials 12, 763 (2000),
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