Non-toxic cell permeable fluorescent dye that reacts with amine compounds. Readily accumulates inside viable cells. Hardly leaks from cells when covalently attached to intracellular proteins. Hydrolyzed CFSE emits fluorescence (Ex(max): 495nm; Em(max): 519nm). Therefore, CFSE is utilized for the long-term observation of cell activities by fluorescent microscopy as well as for viable cell detection.
Product Details
| Alternative Name: | 5(6)-(N-Succinimidyloxycarbonyl)-3',6',0,0'-diacetylfluorescein, 5(6)-Carboxyfluorescein diacetate, N-succinimidyl ester- |
| |
| Formula: | C29H19NO11 |
| |
| MW: | 557.5 |
| |
| CAS: | 150347-59-4 |
| |
| Purity: | ≥95% (HPLC) |
| |
| Appearance: | White or slightly yellow powder. |
| |
| Solubility: | Soluble in DMSO, dimethyl formamide or methanol. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Protect from light. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
Product Literature References
Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes: S.W. Kamau, et al.; BMC Pharmacol.
1, 1 (2001),
Abstract;
Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question: E.J. Suchin, et al.; J. Immunol.
166, 973 (2001),
Abstract;
Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution: A.B. Lyons; J. Immunol. Methods
243, 147 (2000),
Abstract;
Flow cytometric cell division tracking using nuclei: J. Hasbold & P.D. Hodgkin; Cytometry
40, 230 (2000),
Abstract;
Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro: H.E. Gruber, et al.; Biotech. Histochem.
75, 118 (2000),
Abstract;
Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven: B.F. de St Groth, et al.; Immunol. Cell Biol.
77, 530 (1999),
Abstract;
The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo: J. Mintern, et al.; Immunol. Cell Biol.
77, 539 (1999),
Abstract;
Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester: A.B. Lyons; Immunol. Cell Biol.
77, 509 (1998),
Abstract;
A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy: S. Oehen, et al.; J Immunol Methods
207, 33 (1997),
Abstract;
Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function: L.S. De Clerck, et al.; J. Immunol. Methods
172, 115 (1994),
Abstract;
New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy: S.A. Weston & C.R. Parish; J. Immunol. Methods
133, 87 (1990),
Abstract;
