Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

2',7'-Dichlorodihydrofluorescein diacetate

Peroxynitrite indicator
ALX-610-022-M050 50 mg 61.00 USD
Do you need bulk/larger quantities?
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=59,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.

Product Details

Alternative Name:DCDHF diacetate, 2',7'-Dichlorofluorescein diacetate
Purity:≥95% (HPLC)
Appearance:White to light orange-brown solid.
Solubility:Soluble in DMSO (≥25mg/ml), 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately dilute with PBS, pH 7.2, to the desired concentration.
Regulatory Status:RUO - Research Use Only
Please mouse over

Product Literature References

Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: C.Y. Hsieh, et al.; Int. J. Mol. Sci. 24, 3561 (2023), Abstract;
Bisdemethoxycurcumin-mediated Attenuation of Apoptosis Prevents Gentamicin-induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: T.Y. Kang, et al.; In Vivo 36, 1095 (2022), Abstract;
Effects Mediated by Dimethyl Fumarate on In Vitro Oligodendrocytes: Implications in Multiple Sclerosis: C. Guerriero, et al.; Int. J. Mol. Sci. 23, 3615 (2022), Abstract;
Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles: B. Van den Broek, et al.; Cell. Commun. Signal. 20, 58 (2022), Abstract; Full Text
Peroxisome proliferator-activated receptor-δ-mediated upregulation of catalase helps to reduce ultraviolet B-induced cellular injury in dermal fibroblasts: J. Hur, et al.; J. Dermatol. Sci. 923, 170 (2021), Abstract;
Lotus Seedpod Extracts Reduced Lipid Accumulation and Lipotoxicity in Hepatocytes: Y.T. Liu, et al.; Nutrients 11, 2895 (2019), Abstract; Full Text
CISD2 inhibition overcomes resistance to sulfasalazine-induced ferroptotic cell death in head and neck cancer: E.H. Kim, et al.; Cancer Lett. 432, 180 (2018), Abstract;
Heme oxygenase-1 mediates BAY 11-7085 induced ferroptosis: L.C. Chang, et al.; Cancer Lett. 416, 124 (2018), Abstract;
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999), Abstract;
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999), Abstract;
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997), Abstract;
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994), Abstract;

Product Toolbox


Certificate of Analysis


By catalog section:


Technical Service
Customer Service

Related Literature

Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Download as PDF

All new literature pieces