Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=59,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.
Product Details
| Alternative Name: | DCDHF diacetate, 2',7'-Dichlorofluorescein diacetate |
| |
| Formula: | C24H16O7Cl2 |
| |
| MW: | 487.3 |
| |
| CAS: | 4091-99-0 |
| |
| Purity: | ≥95% (HPLC) |
| |
| Appearance: | White to light orange-brown solid. |
| |
| Solubility: | Soluble in DMSO (≥25mg/ml), 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately dilute with PBS, pH 7.2, to the desired concentration. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
Product Literature References
Effects Mediated by Dimethyl Fumarate on In Vitro Oligodendrocytes: Implications in Multiple Sclerosis: C. Guerriero, et al.; Int. J. Mol. Sci.
23, 3615 (2022),
Abstract;
Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles: B. Van den Broek, et al.; Cell. Commun. Signal.
20, 58 (2022),
Abstract;
Full Text
Peroxisome proliferator-activated receptor-δ-mediated upregulation of catalase helps to reduce ultraviolet B-induced cellular injury in dermal fibroblasts: J. Hur, et al.; J. Dermatol. Sci.
923, 170 (2021),
Abstract;
Lotus Seedpod Extracts Reduced Lipid Accumulation and Lipotoxicity in Hepatocytes: Y.T. Liu, et al.; Nutrients
11, 2895 (2019),
Abstract;
Full Text
CISD2 inhibition overcomes resistance to sulfasalazine-induced ferroptotic cell death in head and neck cancer: E.H. Kim, et al.; Cancer Lett.
432, 180 (2018),
Abstract;
Heme oxygenase-1 mediates BAY 11-7085 induced ferroptosis: L.C. Chang, et al.; Cancer Lett.
416, 124 (2018),
Abstract;
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol.
301, 367 (1999),
Abstract;
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med.
27, 146 (1999),
Abstract;
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide
1, 145 (1997),
Abstract;
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med.
16, 149 (1994),
Abstract;
