Useful reagent for evaluating LPS binding and cell activation in white blood cells.
Product Details
| Alternative Name: | Lipopolysaccharide from Salmonella abortus equi S-form |
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| Source: | Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered. |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double-distilled water. |
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| Purity: | Absence of dectectable protein or DNA contaminants with agonistic TLR activity. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Handling: | Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel. After opening, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Note: As the biotinylation reagent and standard biotinylation protocol favors protein/peptide (contaminants) about 1000-fold over sugar as a target, it is mandatory to use >99.9% pure LPS to exclude the biotinylation of contaminants. These are usually present in many commercial LPS preparations and would lead to misleading observations of the tracking/staining with the reagents. TLRGRADE® LPS fulfills such high quality requirements and the biotinylation procedure is chosen to preserve TLR4 (Toll-like receptor 4) binding and cellular activation using TLRGRADE® LPS-Biotin.
For the unconjugated LPS, please see ALX-581-009). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Increased TNF expression in CD43++ murine blood monocytes: B. Burke, et al.; Immunol. Lett.
118, 142 (2008),
Abstract;
Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood: M.K. Brunialti, et al.; Cytometry
50, 14 (2002),
Abstract;
Full Text
Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients: R. Salomao, et al.; J. Endotoxin Res.
8, 371 (2002),
Abstract;
Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein: D. Diament, et al.; Infect. Immun.
70, 1677 (2002),
Abstract;
Full Text
Newer aspects of the chemsitry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component: C. Galanos, et al.; Int. Rev. Biochem. 14, 239 (1977),
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