TLR4-specific activation as shown in TLR4-deficient cells
Convenient and stable ready-to-use solution
Highly cited
TLRGRADE® LPS is commonly used to active TLR4, trigger inflammatory signaling, and co-stimulate the immune response, both in vitro and in vivo.
Product Details
Alternative Name:
Lipopolysaccharide from E. coli, Serotype O55:B5 S-form
Biological Activity:
Strong activator of Toll-like receptor (TLR) 4. It does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4-deficient mice. No further re-extraction required. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
Source:
Smooth (S)-form LPS isolated and purified from E. coli 055:B5 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.
Concentration:
1mg/ml
Formulation:
Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
Purity:
Absence of detectable protein or DNA contaminants with agonistic TLR activity.
Shipping:
Ambient
Long Term Storage:
+4°C
Use/Stability:
Stable for at least 1 year after receipt when stored at +4°C.
Handling:
Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
Regulatory Status:
RUO - Research Use Only
Figure: TLR4-dependent proliferation of mouse splenocytesMethod: 4x105 mouse spleen cells were incubated in 0.2ml per well with 10µg/ml LPS or with medium only. Proliferation was determined in triplicates by 3H-thymidine incorporation.
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Product Literature References
An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity: F. Borriello, et al.; Cell 185, 614 (2022), Abstract;
Inhibition of transcription factor NFAT activity in activated platelets enhances their aggregation and exacerbates gram-negative bacterial septicemia: V. Poli, et al.; Immunity 55, 224 (2022), Abstract;
Macrophages Are Polarized toward an Inflammatory Phenotype by Their Aged Microenvironment in the Human Skin: L. Gather, et al.; J. Invest. Dermatol. 142, 3136 (2022), Abstract;
Next-Generation Diprovocims with Potent Human and Murine TLR1/TLR2 Agonist Activity That Activate the Innate and Adaptive Immune Response: M.H. Yang, et al.; J. Med. Chem. 65, 9230 (2022), Abstract;
Non-canonical anti-cancer, anti-metastatic, anti-angiogenic and immunomodulatory PDT potentials of water soluble phthalocyanine derivatives with imidazole groups and their intracellular mechanism of action: F. Ayaz, et al.; Photodiagnosis Photodyn. Ther. 39, 103035 (2022), Abstract;
Protection against severe infant lower respiratory tract infections by immune training: Mechanistic studies: N.M. Troy, et al.; J. Allergy Clin. Immunol. (2022), Abstract;
Specific immunosuppressive role of nanodrugs targeting calcineurin in innate myeloid cells: M. Colombo, et al.; iScience 25, 105042 (2022), Abstract; Full Text
TAK1 protein kinase activity is required for TLR signalling and cytokine production in myeloid cells: M. Rodrigues, et al.; Biochem. J. 479, 1891 (2022), Abstract; Full Text
The HIFα-stabilizing drug Roxadustat increases the number of renal Epo-producing Sca-1+ cells: A. Jatho, et al.; Cells 11, 753 (2022), Abstract; Full Text
CRIg on liver macrophages clears pathobionts and protects against alcoholic liver disease: Y. Duan, et al.; Nat. Commun. 12, 7172 (2021), Abstract;
DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization: L. Liu, et al.; Cell Death Dis. 12, 500 (2021), Abstract; Full Text
De novo synthesized polyunsaturated fatty acids operate as both host immunomodulators and nutrients for Mycobacterium tuberculosis: T. Laval, et al.; Elife 10, e71946 (2021), Abstract; Full Text
Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca2+ mobilization and the inflammatory activity of dendritic cells: L. Marongiu, et al.; Sci. Signal. 14, 2120 (2021), Abstract;
Neural stem cells traffic functional mitochondria via extracellular vesicles: L.P. Jametti, et al.; PLoS Biol. 19, 1371 (2021), Abstract;
Overlapping but disparate inflammatory and immunosuppressive responses to SARS-CoV-2 and bacterial sepsis: an immunological time course analysis: T. Loftus, et al.; Front. Immunol. 12, 792448 (2021), Abstract; Full Text
Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosis: K. Phulphager, et al.; Cell Rep. 34, 108826 (2021), Abstract;
The development of novel glucocorticoid receptor antagonists: From rational chemical design to therapeutic efficacy in metabolic disease models: J. Kroon, et al.; Pharmacol. Res. 168, 105588 (2021), Abstract;
The interferon landscape along the respiratory tract impacts the severity of COVID-19: B. Sposito, et al.; Cell 184, 4953 (2021), Abstract; Full Text
Zinc-dependent histone deacetylases drive neutrophil extracellular trap formation and potentiate local and systemic inflammation: V. Poli, et al.; iScience 24, 103256 (2021), Abstract; Full Text
CD40L membrane retention enhances the immunostimulatory effects of CD40 ligation: T. Elmetwali, et al.; Sci. Rep. 10, 342 (2020), Abstract; Full Text
Differential regulation of hepatic physiology and injury by the TAM receptors Axl and Mer: A. Zagorska, et al.; Life Sci. Alli. 3, e202000694 (2020), Abstract; Full Text
G-CSF and G-CSFR Induce a Pro-Tumorigenic Macrophage Phenotype to Promote Colon and Pancreas Tumor Growth: I. Karagiannidis, et al.; Cancers (Basel) 12, 2868 (2020), Abstract; Full Text
GM-CSF induces noninflammatory proliferation of microglia and disturbs electrical neuronal network rhythms in situ: H. Dikmen, et al.; J. Neuroinflammation 17, 235 (2020), Abstract; Full Text
Inflammasomes within hyperactive murine dendritic cells stimulate long-lived T cell-mediated anti-tumor immunity: D. Zhivaki, et al.; Cell Rep. 33, 108381 (2020), Abstract; Full Text
Vascular miR-181b controls tissue factor-dependent thrombogenicity and inflammation in type 2 diabetes: M. Witkowski, et al.; Cardiovasc. Diabetol. 19, 20 (2020), Abstract; Full Text
Baltic amber teething necklaces: could succinic acid leaching from beads provide anti-inflammatory effects?: M.D. Nissen, et al.; BMC Complement. Altern. Med. 19, 162 (2019), Abstract;
Basophil-derived tumor necrosis factor can enhance survival in a sepsis model in mice: A. Pilponsky, et al.; Nat. Immunol. 20, 129 (2019), Abstract; Full Text
Human neutrophils depend on extrinsic factors produced by monocytes for their survival response to TLR4 stimulation: S. SenGupta, et al.; Innate Immun. 25, 473 (2019), Abstract; Full Text
Monocyte procoagulant responses to anthrax peptidoglycan are reinforced by proinflammatory cytokine signaling: N.I. Popescu, et al.; Blood Adv. 3, 2436 (2019), Abstract; Full Text
The Role of Maternally Acquired Antibody in Providing Protective Immunity Against Nontyphoidal Salmonella in Urban Vietnamese Infants: A Birth Cohort Study: R. de Alwis, et al.; J. Infect Dis. 219, 295 (2019), Abstract; Full Text
Anti-TLR2 antibody triggers oxidative phosphorylation in microglia and increases phagocytosis of β-amyloid: A. Rubio-Araiz, et al.; J. Neuroinflammation 15, 247 (2018), Abstract; Full Text
Mouse LIMR3/CD300f is a negative regulator of the antimicrobial activity of neutrophils: K. Ueno, et al.; Sci. Rep. 8, 17406 (2018), Abstract; Full Text
Mycobacterial Phenolic Glycolipids Selectively Disable TRIF-Dependent TLR4 Signaling in Macrophages: R. Oldenburg, et al.; Front. Immunol. 9, 2 (2018), Abstract; Full Text
Soluble mucus component CLCA1 modulates expression of leukotactic cytokines and BPIFA1 in murine alveolar macrophages but not in bone marrow-derived macrophages: N.A. Erickson, et al.; Histochem. Cell Biol. 149, 619 (2018), Abstract; Full Text
Inhibition of SIK2 and SIK3 during differentiation enhances the anti-inflammatory phenotype of macrophages: N.J. Darling, et al.; Biochem. J. 474, 521 (2017), Abstract; Full Text
The Syk-NFAT-IL-2 Pathway in Dendritic Cells Is Required for Optimal Sterile Immunity Elicited by Alum Adjuvants: H.J. Khameneh, et al.; J. Immunol. 198, 196 (2017), Abstract; Full Text
Dynorphin 1-17 and Its N-Terminal Biotransformation Fragments Modulate Lipopolysaccharide-Stimulated Nuclear Factor-kappa B Nuclear Translocation, Interleukin-1beta and Tumor Necrosis Factor-alpha in Differentiated THP-1 Cells: S.S. Fazalul Rahiman, et al.; PLoS One 11, e0153005 (2016), Application(s): Cell culture, Abstract; Full Text
Enhancing the anti-inflammatory activity of chalcones by tuning the Michael acceptor site: H. Rucker, et al.; Org. Biomol. Chem. 13, 3040 (2015), Abstract;
Lipopolysaccharide-induced multinuclear cells: increased internalization of polystyrene beads and possible signals for cell fusion: M. Nakanishi-Matsui, et al.; Biochem. Biophys. Res. Commun. 440, 611 (2013), Abstract;
Lipopolysaccharide induces multinuclear cell from RAW264. 7 line with increased phagocytosis activity: M. Nakanishi-Matsui, et al.; Biochem. Biophys. Res. Commun. 425, 144 (2012), Abstract;
Similarities and differences of innate immune responses elicited by smooth and rough LPS: I. Zanoni, et al.; Immunol. Lett. 142, 41 (2012), Abstract;
Stimulation of TLR4 by recombinant HSP70 requires structural integrity of the HSP70 protein itself: M. Luong, et al.; J. Inflamm (Lond) 9, 11 (2012), Abstract; Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;
CD14 controls the LPS-induced endocytosis of Toll-like receptor 4: I. Zanoni, et al.; Cell 147, 868 (2011), Abstract;
Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality: E. Marchlik, et al.; J. Leukoc. Biol. 88, 1171 (2010), Abstract;
R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006), Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;
Rough (R)-form LPS isolated and purified from E. coli R515 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS, isolated and purified from Salmonella minnesota R595 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS isolated and purified from E. coli EH100 (Ra-mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS isolated and purified from E. coli J5 (Rc mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
Isolated from E. coli, SerotypeR515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, SerotypeR515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
