TLR4-specific activation as shown in TLR4-deficient cells
Convenient and stable ready-to-use solution
Highly cited
TLRGRADE® LPS is commonly used to active TLR4, trigger inflammatory signaling, and co-stimulate the immune response, both in vitro and in vivo.
Product Details
Alternative Name:
Lipopolysaccharide from E. coli, Serotype O55:B5 S-form
Biological Activity:
Strong activator of Toll-like receptor (TLR) 4. It does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4-deficient mice. No further re-extraction required. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
Source:
Smooth (S)-form LPS isolated and purified from E. coli 055:B5 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.
Concentration:
1mg/ml
Formulation:
Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
Purity:
Absence of detectable protein or DNA contaminants with agonistic TLR activity.
Shipping:
Ambient Temperature
Long Term Storage:
+4°C
Use/Stability:
Stable for at least 1 year after receipt when stored at +4°C.
Handling:
Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
Regulatory Status:
RUO - Research Use Only
Figure: TLR4-dependent proliferation of mouse splenocytesMethod: 4x105 mouse spleen cells were incubated in 0.2ml per well with 10µg/ml LPS or with medium only. Proliferation was determined in triplicates by 3H-thymidine incorporation.
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Product Literature References
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Rough (R)-form LPS isolated and purified from E. coli R515 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS, isolated and purified from Salmonella minnesota R595 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS isolated and purified from E. coli EH100 (Ra-mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Rough (R)-form LPS isolated and purified from E. coli J5 (Rc mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
Isolated from E. coli, SerotypeR515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, SerotypeR515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF
