Replaces Prod. #: ALX-581-001
Product Details
| Alternative Name: | Lipopolysaccharide from E. coli, Serotype O55:B5 S-form |
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| Source: | Smooth (S)-form LPS isolated and purified from E. coli 055:B5 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water. |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water. |
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| Purity: | Absence of detectable protein or DNA contaminants with agonistic TLR activity. |
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| Activity: | Specific activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies. |
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| Shipping: | Ambient |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at +4°C. |
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| Handling: | Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: TLR4-dependent proliferation of mouse splenocytesMethod: 4x105 mouse spleen cells were incubated in 0.2ml per well with 10µg/ml LPS or with medium only. Proliferation was determined in triplicates by 3H-thymidine incorporation.
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