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LPS from Salmonella minnesota R595 (Re) (TLRGRADE®) (Ready-to-Use)

TLR4 activator
ALX-581-008-L001 1 ml 177.00 USD
ALX-581-008-L002 2 ml 292.00 USD
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Product Details

Alternative Name:Lipopolysaccharide from Salmonella minnesota R595 (Re)
Source:Rough (R)-form LPS, isolated and purified from Salmonella minnesota R595 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
Purity:Absence of detectable protein or DNA contaminants with agonistic TLR activity.
Activity:Strong activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
Shipping:Ambient Temperature
Long Term Storage:+4°C
Use/Stability:Stable for at least 1 year after receipt when stored at +4°C.
Handling:Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
Regulatory Status:RUO - Research Use Only
Lipid A from E. coli, Serotype R515 (Re) (TLRGRADE) (Ready-to-Use) image
Figure: Activation of macrophages from TLR4 wild type compared to TLR4 deficient mice by LPS and Lipid A from Enzo. Lipid A or LPS concentrations, which induced maximal activation of TLR4 wild type mouse macrophages, were also applied to TLR4 deficient mouse macrophages. 10 units of IL-6 correspond to the detection limit of the IL-6 ELISA.
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Lipid A from E. coli, Serotype R515 (Re) (TLRGRADE) (Ready-to-Use) image

Product Literature References

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