Replaces Prod. #: ALX-581-204
Product Details
| Alternative Name: | Lipopolysaccharide from E. coli, Serotype R515 (Re) |
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| Source: | Rough (R)-form LPS isolated and purified from E. coli R515 (Re mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water. |
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| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water. |
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| Purity: | Absence of detectable protein or DNA contaminants with agonistic TLR activity. |
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| Activity: | Strong activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required.Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at +4°C. |
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| Handling: | Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes Use must be restricted to qualified personnel. Keep sterile. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Activation of macrophages from TLR4 wild type compared to TLR4 deficient mice by LPS and Lipid A from Enzo. Lipid A or LPS concentrations, which induced maximal activation of TLR4 wild type mouse macrophages, were also applied to TLR4 deficient mouse macrophages. 10 units of IL-6 correspond to the detection limit of the IL-6 ELISA.
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