Figure 2: Netrin-1 (human):Fc (human) (rec.) (Prod. No. AG-40B-0075) triggers a DCC-dependent phosphorylation of ERK1/2.
Method: HEK 293 cells (Cont.) or HEK 293 expressing the Netrin-1 receptor DCC (DCC) were incubated with Netrin-1 (h):Fc (h) (5nM) for the indicated time points (shown in minutes). Antibodies againts DCC, total ERK1/2 or phospho ERK1/2 were used, and visualized with a chemiluminescence detection system.
Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon, France.
Figure: Netrin-1 (human):Fc (human) (rec.) (Prod. No. ALX-522-455) induces outgrowth of the commisural axon.
Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody.
Picture courtesy of Dr Véronique Corset, Centre Léon Bérard, Lyon
