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Netrin-1 (human):Fc (human), (recombinant)



ALX-522-455-C010	10 µg	515.00 USD
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Product Specification

MW:	~90kDa (SDS-PAGE)

Source:	Produced in HEK 293 cells. The Fc portion of human IgG1 is fused to the C-terminus of the human netrin-1 (aa 25-604).

UniProt ID:	O95631

Concentration:	0.1mg/ml after reconstitution.

Formulation:	Lyophilized. From PBS containing trehalose and BRIJ 35.

Purity:	≥95% (SDS-PAGE)

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Lonza).

Species reactivity:	Human, Mouse, Rat

Specificity:	Binds to human, mouse and rat UNC5B.

Biological Activity:	Induces axon outgrowth.

Reconstitution:	Reconstitute with 100µl sterile distilled water. PBS containing at least 0.1% BSA should be used for further dilutions.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Regulatory Status:	RUO - Research Use Only

Netrin-1 (human):Fc (human), (recombinant) image

Figure 2: Netrin-1 (human):Fc (human) (rec.) (Prod. No. AG-40B-0075) triggers a DCC-dependent phosphorylation of ERK1/2.
Method: HEK 293 cells (Cont.) or HEK 293 expressing the Netrin-1 receptor DCC (DCC) were incubated with Netrin-1 (h):Fc (h) (5nM) for the indicated time points (shown in minutes). Antibodies againts DCC, total ERK1/2 or phospho ERK1/2 were used, and visualized with a chemiluminescence detection system.
Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon, France.

Figure: Netrin-1 (human):Fc (human) (rec.) (Prod. No. ALX-522-455) induces outgrowth of the commisural axon.
Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody.
Picture courtesy of Dr Véronique Corset, Centre Léon Bérard, Lyon

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