Product Specification
| Alternative Name: | Gastric cancer antigen Zg4, N-terminal fragment of NUCB2, NEFA/nucleobindin2-encoded satiety- and fat-influencing protein |
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| MW: | ~8kDa |
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| Source: | Recombinant human nesfatin-1 (aa 25-106) produced in E. coli. |
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| UniProt ID: | P80303 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Lonza). |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake. |
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General Literature References
Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature
443, 709 (2006),
Abstract;
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