Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor. Note: For some TNF ligands, such as FasL (mouse), activated by further oligomerization (> hexamer), the presence of TNC is not sufficient and ligand crosslinking using the "Enhancer for Ligands" (Prod. No. ALX-804-034) is required.
Product Details
| Alternative Name: | Apo-3L, TNFSF 12 |
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| MW: | ~30kDa |
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| Source: | Produced in HEK 293 cells. The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG®-tag. |
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| UniProt ID: | O54907 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
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| Specificity: | Binds to Fn14 (mouse):Fc (human) (rec.) (Prod. No. ALX-522-036) |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to mouse Fn14. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
Enforced covalent trimerization increases the activity of the TNF ligand family members TRAIL and CD95L: D. Berg, et al.; Cell Death Differ.
14, 2021 (2007),
Abstract;
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