Product Details
| Alternative Name: | Interleukin-33, NF-HEV, IL-1F11 |
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| MW: | ~16kDa |
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| Source: | Produced in E. coli. Untagged rat IL-33 (aa 109-264). |
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| UniProt ID: | Q66H70 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Cape Cod Associates). |
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| Reconstitution: | Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | IL-33 is a member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270). |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis: Lane 1: MW Marker; Lane 2: 1.0μg of purified IL-33 (rat), (recombinant).
Western Blot analysis: Lane 1: MW Marker; Lane 2: 100ng of purified IL-33 (rat), (recombinant) protein probed with IL-33 pAb (Prod. no. ALX-210-933) at 0.5μg/ml.
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Product Literature References
Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation: A.M. Küchler, et al.; Am. J. Pathol.
173, 1229 (2008),
Abstract;
General Literature References
IL-33 and ST2 comprise a critical biomechanically induced and cardioprotective signaling system: S. Sanada, et al.; J. Clin. Invest.
117, 1538 (2007),
Abstract;
Full Text
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity
23, 479 (2005),
Abstract;
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