Produced in HEK 293 cells. The extracellular domain of mouse netrin-1 (aa 27-604) is fused at the C-terminus to a FLAG®-tag.
UniProt ID:
O09118
Concentration:
0.1mg/ml after reconstitution.
Formulation:
Lyophilized. Contains PBS.
Purity:
≥90% (SDS-PAGE)
Purity Detail:
Purified by multi-step chromatography.
Endotoxin Content:
<0.1EU/µg purified protein (LAL test; Bio Whittaker).
Species reactivity:
Human, Mouse
Specificity:
Binds to human and mouse UNC5B.
Applications:
ELISA
Application Notes:
ELISA: binds to UNC5B.
Reconstitution:
Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least 6 months after receipt when stored at -20°C.
Handling:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:
The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis.
Technical Info/Product Notes:
FLAG is a registered trademark of Sigma-Aldrich Co.
Regulatory Status:
RUO - Research Use Only
Product Literature References
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development: T. Tu, et al.; Cell Res. 25, 275 (2015), Abstract; Full Text
General Literature References
Netrin-1: when a neuronal guidance cue turns out to be a regulator of tumorigenesis: P. Mehlen & C. Furne; Cell. Mol. Life Sci. 62, 2599 (2005), (Review), Abstract;
Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer 4, 978 (2004), (Review), Abstract;
Produced in HEK 293 cells. The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a FLAG®-tag., ≥90% (SDS-PAGE), ELISA | Print as PDF
