Product Details
| MW: | ~80kDa |
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| Source: | Produced in HEK 293 cells. The extracellular domain of mouse netrin-1 (aa 27-604) is fused at the C-terminus to a FLAG®-tag. |
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| UniProt ID: | O09118 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse UNC5B. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to UNC5B. |
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| Reconstitution: | Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development: T. Tu, et al.; Cell Res.
25, 275 (2015),
Abstract;
Full Text
General Literature References
Netrin-1: when a neuronal guidance cue turns out to be a regulator of tumorigenesis: P. Mehlen & C. Furne; Cell. Mol. Life Sci.
62, 2599 (2005), (Review),
Abstract;
Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer
4, 978 (2004), (Review),
Abstract;
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