Product Details
| Alternative Name: | Tumor necrosis factor α, TNFSF 2 |
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| MW: | ~48kDa (calculated), ~52kDa (observed) |
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| Source: | Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa). |
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| UniProt ID: | P01375 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that TNFα induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer). |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis of Fc (human):TNF-α (soluble) (human), (recombinant): Lane 1: MW Marker, Lane 2: 1µg TNF-α.
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General Literature References
Life and death decisions: secondary complexes and lipid rafts in TNF receptor family signal transduction: J.R. Muppidi, et al.; Immunity
21, 461 (2004),
Abstract;
