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TL1A (soluble) (human), (recombinant)

ALX-522-084-C010 10 µg 535.00 USD
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Product Details

Alternative Name:TNFSF 15, VEGI
MW:~20kDa (SDS-PAGE).
Source:Produced in E. coli. The extracellular domain of human TL1A (aa 93-251) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
UniProt ID:O95150
Concentration:0.1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥85% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).
Specificity:Binds human DR3 and DcR3.
Applications:ELISA, Flow Cytometry
Application Notes:Flow Cytometry: use together with Enhancer for Ligands (Prod. No. ALX-804-034) to detect human DR3 and DcR3.
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:TL1A (TNFSF15), a ligand belonging to the tumor necrosis factor (TNF) family, is expressed predominantly by endothelial cells and monocytes. TL1a is upregulated by the proinflammatory cytokines TNF and IL-1 and also by immune complexes (IC). TL1 functions in T cell costimulation and Th1 polarization. On activated T cells, TL1A functions specifically via its surface-bound receptor DR3, (a member of the death-domain containing TNF receptor family) to promote cell survival and secretion of proinflammatory cytokines (1). The secreted decoy receptor 3 (DcR3), a soluble protein of the tumor necrosis factor receptor (TNFR) superfamily blocks the action of TL1A. TL1A can be released to circulate as a homotrimeric soluble form. Activation of DR3 by TL1A induced the formation of a signaling complex containing TRADD, TRAF2, and RIP and activated the NF-κB and the ERK, JNK, and p38 mitogen-activated protein kinase pathways. The TL1A/DR3 pathway plays an important role in Th1-mediated intestinal diseases, such as Crohn’s disease.
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
Regulatory Status:RUO - Research Use Only

General Literature References

Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis: M.A. Cassatella, et al.; J. Immunol. 178, 7325 (2007), Abstract;
Identification of naturally secreted soluble form of TL1A, a TNF-like cytokine: S. Kim & L. Zhang; J. Immunol. Methods 298, 1 (2005), Abstract;
Enhanced adhesion of monocytes via reverse signaling triggered by decoy receptor 3: M.J. Hsu, et al.; Exp. Cell Res. 292, 241 (2004), Abstract;
Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease: G. Bamias, et al.; J. Immunol. 171, 4868 (2003), Abstract;
TL1A-induced NF-kappaB activation and c-IAP2 production prevent DR3-mediated apoptosis in TF-1 cells: L. Wen, et al.; J. Biol. Chem. 278, 39251 (2003), Abstract; Full Text
TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator: T.S. Migone, et al.; Immunity 16, 479 (2002), Abstract;

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