Product Specification
| Alternative Name: | TNFRSF 17, B cell maturation protein, CD269 |
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| MW: | ~35kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of mouse BCMA (aa 1-46) is fused at the C-terminus to a linker peptide (2 aa) and the Fc portion of human IgG1. |
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| UniProt ID: | O88472 |
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| Gene/Protein Identifier: | A000374 (UCSD Signaling Gateway ID) |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Specificity: | Binds mouse and human BAFF and human APRIL. |
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| Applications: | ELISA, Flow Cytometry, FUNC
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| Application Notes: | ELISA: binds to BAFF.
Flow Cytometry: Detection of membrane-bound mouse BAFF and human APRIL in combination with fluorescently labelled Ab to human IgG1. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that BCMA blocks the binding of mouse BAFF and human APRIL to their receptors BCMA and TACI, inhibiting BAFF and APRIL-mediated splenocyte survival. |
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| Regulatory Status: | RUO - Research Use Only |
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Inhibition of rhsBAFF-mediated splenocyte survival.
Figure: rmBCMA:Fc inhibits rhsBAFF-mediated activation of freshly isolated splenocytes. Concentration of rmBCMA:Fc (Prod. Nr.
ALX-522-079) to achieve complete inhibition depends on the concentration of rhsBAFF (Prod. Nr.
ALX-522-025) used. A ratio (rhsBAFF:rmBCMA:Fc) 1:50 and higher is recommended.
Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS, and B cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone, with the indicated concentration of rhsBAFF alone or in the presence of an excess of rmBCMA:Fc (50µg/ml). After three days in culture, cells were again analyzed on FACS with the saved setting. Splenocytes survival index (=ratio of %living/%dead cells) was calculated and plotted."
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Product Literature References
Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med.
194, 1691 (2001),
Abstract;
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