Product Details
| Alternative Name: | CD154, TNFSF 5, gp39 |
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| MW: | ~58kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa). |
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| UniProt ID: | P27548 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse CD40. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to human CD40:COMP at 10-100 ng/ml. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that Fc (human):CD40L (mouse) stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Proliferation of mouse B cells induced by Fc (human):CD40L, Soluble (mouse) (rec.) (Prod. No.
ALX-522-072).
Method: Splenocytes from Balb/c mice were purified on B220 magnetic beads using the MACS system. The resulting B cells were put in culture at a cell density of 70’000 cells/well in a 96-well plate. Cells were activated with the indicated concentrations of Fc (human):CD40L, Soluble (mouse) (rec.). After 36 hours, 0.5µCi of 3H-Thymidine/well was added. Cells were pulsed for 10 hours, then freeze-thawed, harvested and counted."
SDS-PAGE analysis of Fc (human):CD40L (soluble) (mouse), (recombinant) (Prod. no. ALX-522-072): Lane 1: MW Marker, Lane 2: 1 µg.
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Product Literature References
Reciprocal CD40 signals through p38MAPK and ERK-1/2 induce counteracting immune responses: R.K. Mathur, et al.; Nat. Med.
10, 540 (2004),
Abstract;
