Figure: rhBAFF-R:Fc inhibits rhsBAFF-mediated activation of freshly isolated mouse splenocytes. Concentration of rhBAFF-R:Fc required to achieve complete inhibition depends on the concentration of rhsBAFF (Prod. No.
ALX-522-025) used. A ratio (rhsBAFF:rhBAFF-R:Fc) 1:50 and higher is recommended.
Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed on FACS, and the B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media only, with the indicated concentration of rhsBAFF (Prod. No.
ALX-522-025) alone or in the presence of excess rhsBAFF-R:Fc (+5µg/ml). After 3 days in culture, cells were again analyzed on FACS with the saved settings. Ratio of % living/% dead cells was calculated and plotted."