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TACI (mouse):Fc (human), (recombinant)

ALX-522-053-C050 50 µg 417.00 USD
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Product Specification

Alternative Name:TNFRSF 13B, Transmembrane activator and CAML interactor, CD267
Source:Produced in HEK 293 cells. The extracellular domain of mouse TACI (aa 1-86) is fused to the Fc portion of human IgG1.
UniProt ID:Q9ET35
Gene/Protein Identifier:A002248 (UCSD Signaling Gateway ID)
Concentration:1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥90% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).
Specificity:Binds human (weak) and mouse BAFF and APRIL.
Application Notes:ELISA: binds to BAFF.
Reconstitution:Reconstitute with 50µl sterile water.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Technical Info/Product Notes:Historical data has shown that TACI receptor inhibits rmsBAFF mediated B cell activation.
Regulatory Status:RUO - Research Use Only
TACI (mouse):Fc (human), (recombinant) Flow Cytometry
Figure: rmTACI:Fc inhibits rmsBAFF-mediated proliferation of freshly isolated mouse B cells. Concentration of rmTACI:Fc required to achieve complete inhibition depends on the concentration of rmsBAFF used. A ratio (rmBAFF:rmTACI:Fc) 1:50 and higher is recommended.
Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed by FACS, and B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone, with 100ng/ml rms BAFF or with 100ng/ml rmsBAFF and a 1:50 excess of rms TACI:Fc (+5µg/ml). After three days in culture, cells were again analyzed by FACS with the saved setting, and % of B cells that survived was calculated.
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TACI (mouse):Fc (human), (recombinant) Flow Cytometry

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