Product Specification
| Alternative Name: | TNFRSF 13B, Transmembrane activator and CAML interactor, CD267 |
| |
| Source: | Produced in HEK 293 cells. The extracellular domain of mouse TACI (aa 1-86) is fused to the Fc portion of human IgG1. |
| |
| UniProt ID: | Q9ET35 |
| |
| Gene/Protein Identifier: | A002248 (UCSD Signaling Gateway ID) |
| |
| Concentration: | 1mg/ml after reconstitution. |
| |
| Formulation: | Lyophilized. Contains PBS. |
| |
| Purity: | ≥90% (SDS-PAGE) |
| |
| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
| |
| Specificity: | Binds human (weak) and mouse BAFF and APRIL. |
| |
| Applications: | ELISA
|
| |
| Application Notes: | ELISA: binds to BAFF. |
| |
| Reconstitution: | Reconstitute with 50µl sterile water. |
| |
| Shipping: | Shipped on Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Technical Info/Product Notes: | Historical data has shown that TACI receptor inhibits rmsBAFF mediated B cell activation. |
| |
Figure: rmTACI:Fc inhibits rmsBAFF-mediated proliferation of freshly isolated mouse B cells. Concentration of rmTACI:Fc required to achieve complete inhibition depends on the concentration of rmsBAFF used. A ratio (rmBAFF:rmTACI:Fc) 1:50 and higher is recommended.
Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed by FACS, and B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone, with 100ng/ml rms BAFF or with 100ng/ml rmsBAFF and a 1:50 excess of rms TACI:Fc (+5µg/ml). After three days in culture, cells were again analyzed by FACS with the saved setting, and % of B cells that survived was calculated.
Please mouse over
Related Products
For Research Use Only. Not for use in diagnostic procedures.
