Product Specification
Alternative Name: | OX40L, TNFSF 4, CD252 |
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MW: | ~28kDa (under reducing conditions). |
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Source: | Produced in HEK 293 cells. The extracellular domain of mouse CD134L (OX40L) (aa 52-198) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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UniProt ID: | P43488 |
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Concentration: | 0.1mg/ml after reconstitution. |
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Formulation: | Lyophilized. Contains PBS. |
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Purity: | ≥90% (SDS-PAGE) |
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Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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Species reactivity: | Human, Mouse
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Specificity: | Binds to human and mouse CD134 (OX40). |
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Applications: | ELISA
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Application Notes: | ELISA: binds to CD134 receptor. Can be used together with anti-FLAG® MAb to detect membrane-bound mouse CD134 (OX40). |
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Reconstitution: | Reconstitute with 100µl sterile water. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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Technical Info/Product Notes: | Historical data has shown that CD40L activates T cell proliferation.
Ligand enhancer ALX-804-034 can be used as an accessory to increase specific activity.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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Regulatory Status: | RUO - Research Use Only |
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General Literature References
Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34: P.R. Baum, et al.; EMBO J.
13, 3992 (1994),
Abstract;