| Alternative Name: | TNFRSF 7, CD27L receptor, T-cell activation antigen CD27 |
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| MW: | ~55kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The cysteine-rich region of human CD27 (aa 1-191) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | P26842 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity Detail: | Purified by single-step affinity chromatography. |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse CD70 (CD27L). |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to CD27L at 1-10 ng/ml.
Can be used together with anti-human IgG1 PAb to detect membrane-bound human and mouse CD70. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | The CD27 receptor (CD27, Tp55) is a member of the tumor necrosis factor receptor (TNF-R) family. CD27 is a type I transmembrane receptor, it contains 3 extracellular cysteine-rich motifs. CD27 is mainly expressed on T and B lymphocytes, and its expression is increased on T cells by the engagement of the T cell receptor complex. Engagement of CD27 induces recruitment of TNF receptor-associated factor 2 (TRAF2) and TRAF3. CD27-CD70 have been reported to be involved in several different immune mechanisms such as T, NK and B lymphocytes interactions and costimulation, early thymic differentiation, autoimmune and viral diseases and apoptosis. |
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| Regulatory Status: | RUO - Research Use Only |
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