Product Details
| Alternative Name: | TNFRSF 17, B cell maturation protein, CD269 |
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| MW: | ~40kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human BCMA (aa 2-54) is fused at the C-terminus to the Fc portion of human IgG1. |
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| UniProt ID: | Q02223 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse BAFF and APRIL. |
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| Applications: | ELISA, Flow Cytometry
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| Application Notes: | ELISA: binds to mouse BAFF).
Flow Cytometry: Detection of membrane-bound mouse BAFF and human APRIL in combination with fluorescently labelled Ab to IgG1. |
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| Reconstitution: | Reconstitute with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data shows that it blocks the binding of BAFF and APRIL to their receptors BCMA and TACI, inhibiting BAFF- and APRIL-mediated B cell activation. |
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| Regulatory Status: | RUO - Research Use Only |
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Schematic structure of human rhBCMA:Fc.
Figure: Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (2). Control (1) are untransfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.Method: 293 cells (5 x 105) expressing surface BAFF vector were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 4µg/ml of BCMA:Fc. After washing in FACS buffer, PE-conjugated antibody to human IgG (SBA) was added and cells were analyzed by flow cytometry.
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Product Literature References
T cells isolated from G-CSF-treated multiple myeloma patients are suitable for the generation of BCMA-directed CAR-T cells: A.M. Battram, et al.; Mol. Ther. Methods Clin. Dev.
26, 207 (2022),
Abstract;
Long-Lived Plasma Cells from Human Small Intestine Biopsies Secrete Immunoglobulins for Many Weeks In Vitro: L. Mesin, et al.; J. Immunol.
187, 2867 (2011),
Abstract;
Blocking the APRIL circuit enhances acute myeloid leukemia cell chemosensitivity: D. Bonci, et al.; Haematologica
93, 1899 (2008),
Abstract;
Full Text
Essential role of antigen-presenting cell-derived BAFF for antibody responses: F. Bergamin, et al.; Eur. J. Immunol.
37, 3100 (2007),
Abstract;
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha: M. Nishio, et al.; Blood
106, 1012 (2005),
Abstract;
Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol.
16, 467 (2004),
Abstract;
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood
100, 2973 (2002),
Abstract;
Full Text
Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med.
194, 1691 (2001),
Abstract;
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol.
167, 6225 (2001),
Abstract;
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med.
192, 1677 (2000),
Abstract;
Full Text
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med.
192, 129 (2000),
Abstract;
Full Text
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