Product Details
| Alternative Name: | THANK, zTNF4, TALL-1, BlyS, TNFSF 13B/20, CD257 |
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| MW: | ~28kDa (SDS-PAGE). |
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| Source: | Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag. |
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| UniProt ID: | Q9Y275 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse (weak) BCMA, TACI and BAFF-R. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to BAFF receptor at 2-100 ng/ml.
Detection of receptors of human soluble BAFF on target cells. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that BAFF mediates splenocyte survival and induces monocyte survival.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 μg BAFF. Bands indicated are immunoreactive with anti-FLAG antibody.
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Product Literature References
RUNX1 Regulates a Transcription Program That Affects the Dynamics of Cell Cycle Entry of Naive Resting B Cells: I. Thomsen, et al.; J. Immunol.
207, 2976 (2021),
Abstract;
Human secretory IgM emerges from plasma cells clonally related to gut memory B cells and targets highly diverse commensals: G. Magri, et al.; Immunity
47, 118 (2017),
Abstract;
Full Text
The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity: F.S. Hoffmann, et al.; J. Immunol.
194, 542 (2015),
Application(s): Detection of TACI-BAFF-APRIL complexes by ELISA,
Abstract;
Full Text
Disturbed B cell homeostasis in newly diagnosed giant cell arteritis and polymyalgia rheumatica: K.S. van der Geest, et al.; Arthritis Rheumatol.
66, 1927 (2014),
Abstract;
Stromal endothelial cells establish a bidirectional crosstalk with chronic lymphocytic leukemia cells through the TNF-related factors BAFF, APRIL and CD40L: M. Cols, et al.; J. Immunol.
188, 6071 (2012),
Application(s): UVEC & MVEC entothelial cell culture,
Abstract;
Full Text
Viral Double-Stranded RNA Triggers Ig Class Switching by Activating Upper Respiratory Mucosa B Cells through an Innate TLR3 Pathway Involving BAFF: W. Xu, et al.; J. Immunol.
181, 276 (2008),
Abstract;
A role for BLyS in the activation of innate immune cells: S.K. Chang, et al.; Blood
108, 2687 (2006),
Abstract;
Full Text
TACI is mutant in common variable immunodeficiency and IgA deficiency: E. Castigli, et al.; Nat. Genet.
37, 829 (2005),
Abstract;
Expression of BLyS and its Receptors in B-Cell Non-Hodgkin Lymphoma: Correlation with Disease Activity and Patient Outcome: A.J. Novak, et al.; Blood
104, 2247 (2004),
Abstract;
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival: A.J. Novak, et al.; Blood
100, 2973 (2002),
Abstract;
Full Text
BAFF is a survival and maturation factor for mouse B cells: A.G. Rolink, et al.; Eur. J. Immunol.
32, 2004 (2002),
Abstract;
Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med.
194, 1691 (2001),
Abstract;
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol.
167, 6225 (2001),
Abstract;
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med.
192, 129 (2000),
Abstract;
Full Text
BAFF mediates survival of peripheral immature B lymphocytes: M. Batten, et al.; J. Exp. Med.
192, 1453 (2000),
Abstract;
Full Text
Total Syntheses of (±)-Aerothionin, (±)-Homoaerothionin, and (±)-Aerophobin-1: S. Nishiyama & S. Yamamura; Bull. Chem. Soc. Jpn.
58, 3453 (1985),
Abstract;
General Literature References
TNFR-associated factor-3 is associated with BAFF-R and negatively regulates BAFF-R-mediated NF-kappa B activation and IL-10 production: L.G. Xu & H.B. Shu; J. Immunol.
169, 6883 (2002),
Abstract;
Full Text
