Product Details
Alternative Name: | OX40L, TNFSF 4, CD252 |
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MW: | ~30kDa (SDS-PAGE). |
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Source: | Produced in HEK 293 cells. The extracellular domain of human CD134L (OX40L) (aa 52-183) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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UniProt ID: | P23510 |
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Concentration: | 0.1mg/ml after reconstitution. |
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Formulation: | Lyophilized. Contains PBS. |
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Purity Detail: | Purified by single-step affinity chromatography. |
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Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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Specificity: | Binds to human CD134 (OX40). |
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Applications: | ELISA
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Application Notes: | ELISA: binds to CD134 receptor). Can be used together with anti-FLAG® MAb to detect membrane-bound human CD134 (OX40). |
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Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -80°C |
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Use/Stability: | Stable for at least 6 months after receipt when stored at -80°C. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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Technical Info/Product Notes: | Historical data indicates that CD134L activates T cell proliferation and delays neutrophil apoptosis.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A novel method for determination of the affinity of protein: protein interactions in homogeneous assays: P. Newton, et al.; J. Biomol. Screen.
13, 674 (2008),
Abstract;
Enhancing the immunostimulatory function of dendritic cells by transfection with mRNA encoding OX40 ligand: J. Dannull, et al.; Blood
105, 3206 (2005),
Abstract;
Functional expression of CD134 by neutrophils: R. Baumann, et al.; Eur. J. Immunol.
34, 2268 (2004),
Abstract;
General Literature References
Functional expression of CD134 by neutrophils: R. Baumann, et al.; Eur. J. Immunol.
34, 2268 (2004),
Abstract;
CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells: H. Akiba, et al.; J. Immunol.
162, 7058 (1999),
Abstract;
CD4 T cell traffic control: in vivo evidence that ligation of OX40 on CD4 T cells by OX40-ligand expressed on dendritic cells leads to the accumulation of CD4 T cells in B follicles: T. Brocker, et al.; Eur. J. Immunol.
29, 1610 (1999),
Abstract;
OX40-Mediated cosignal enhances the maturation of naive human CD4+ T cells into high IL-4-producing effectors: G. Delespesse, et al.; Int. Arch. Allergy Immunol.
118, 384 (1999),
Abstract;
Ox-40 ligand: a potent costimulatory molecule for sustaining primary CD4 T cell responses: I. Gramaglia, et al.; J. Immunol.
161, 6510 (1998),
Abstract;