Product Details
| Alternative Name: | Apo-3L, TNFSF 12 |
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| MW: | ~18-20kDa (SDS-PAGE). |
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| Source: | Produced in E. coli. The extracellular domain of human TWEAK (aa 106-249) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag. |
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| UniProt ID: | O43508 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse Fn14 (TWEAK-R). |
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| Biological Activity: | Kills TWEAK sensitive cells. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to FN14 receptor at 10-100 ng/ml. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that TWEAK has a pro-apoptotic effect on several target cell lines, and it induces angiogenesis and proliferation in endothelial cells.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Apoptosis of TWEAK sensitive cells. Recombinant, human TWEAK exerts its full biological activity in a concentration range of 50-500ng/ml on Kym-1 cells.Method: Kym-1 cells (20’000 cells in 50µl RPMI medium containing 10% fetal calf serum) were incubated with the indicated concentrations of rhsTWEAK for 24 hours at 37°C in a 96 well plate. Concentrations of rhsTWEAK required to kill target cells may vary depending on cell type studied. Cell viability was determined using a PMS/MTS-based cell proliferation assay kit.
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Product Literature References
Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2: A. Annibaldi, et al.; Mol. Cell
69, 566 (2018),
Abstract;
Full Text
Non-canonical NFκB activation promotes chemokine expression in podocytes: L. Valino-Rivas, et al.; Sci. Rep.
6, 28857 (2016),
Application(s): Cell cultures, renal cells,
Abstract;
Full Text
Intraovarian TWEAK/Fn14 ligand-receptor system limits ovarian preovulatory follicles from excessive luteinization: A. De, et al.; Mol. Endocrinol.
20, 2528 (2006),
Abstract;
Full Text
Induction of RANTES by TWEAK/Fn14 interaction in human keratinocytes: L. Jin, et al.; J. Invest. Dermatol.
122, 1179 (2004),
Abstract;
TWEAK/Fn14 interaction stimulates human bronchial epithelial cells to produce IL-8 and GM-CSF: H. Xu, et al.; BBRC
318, 422 (2004),
Abstract;
