Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
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| MW: | ~19kDa (SDS-PAGE). |
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| Source: | Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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| UniProt ID: | P01375 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (Prod. No. ALX-804-034), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to TNF-R2 at 0.1-10 ng. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical lots have demonstrated biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells), and an ED50 of 0.1ng/ml (WEHI 164 cells).
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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