Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
| |
| MW: | ~19kDa (SDS-PAGE). |
| |
| Source: | Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
| |
| UniProt ID: | P01375 |
| |
| Concentration: | 1mg/ml after reconstitution. |
| |
| Formulation: | Lyophilized. Contains PBS. |
| |
| Purity: | ≥95% (SDS-PAGE) |
| |
| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
| |
| Species reactivity: | Human, Mouse
|
| |
| Specificity: | Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (Prod. No. ALX-804-034), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α. |
| |
| Applications: | ELISA
|
| |
| Application Notes: | ELISA: binds to TNF-R2 at 0.1-10 ng. |
| |
| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Technical Info/Product Notes: | Historical lots have demonstrated biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells), and an ED50 of 0.1ng/ml (WEHI 164 cells).
FLAG is a registered trademark of Sigma-Aldrich Co. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Discovery of a Non-competitive TNFR1 Antagonist Affibody with Picomolar Monovalent Potency That Does Not Affect TNFR2 Function: N. Vunnam, et al.; Mol. Pharm.
20, 1884 (2023),
Abstract;
Resveratrol has its antioxidant and anti-inflammatory protective mechanisms decreased in aging: M.A. Santos, et al.; Arch. Gerontol. Geriatr.
107, 104895 (2023),
Abstract;
SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth: Y.Q. Yu, et al.; Cell Death Dis.
13, 52 (2022),
Abstract;
IRF-8/miR-451a regulates M-MDSC differentiation via the AMPK/mTOR signal pathway during lupus development: G. Shi, et al.; Cell Death Discov.
7, 41420 (2021),
Abstract;
Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains: P.R. Elliott, et al.; Cell Rep.
37, 109777 (2021),
Abstract;
The ubiquitin ligase HOIL-1L regulates immune responses by interacting with linear ubiquitin chains: C.G. Diaz, et al.; iScience
24, 103241 (2021),
Abstract;
CD40L membrane retention enhances the immunostimulatory effects of CD40 ligation: T. Elmetwali, et al.; Sci. Rep.
10, 342 (2020),
Abstract;
Full Text
USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination: J. Roedig, et al.; EMBO Rep.
2020, e50163 (2020),
Abstract;
Metformin inhibits pro-inflammatory responses via targeting nuclear factor-κB in HaCaT cells: W. Ba, et al.; Cell Biochem. Funct.
37, 4 (2019),
Abstract;
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell
73, 413 (2019),
Abstract;
Full Text
Proteasome inhibition blocks necroptosis by attenuating death complex aggregation: M. Ali, et al.; Cell Death Dis.
9, 346 (2018),
Abstract;
Full Text
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death: I. Jaco, et al.; Mol. Cell
66, 698 (2017),
Abstract;
Full Text
Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis.
6, e1800 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest): M.K. Hospenthal, et al.; Nat. Protoc.
10, 349 (2015),
Abstract;
Full Text
Small-molecule modulators of the OX40-OX40L costimulatory protein-protein interaction: Y. Song, et al.; Br. J. Pharmacol.
171, 4955 (2014),
Abstract;
De-ubiquitinating proteases USP2a and USP2c cause apoptosis by stabilising RIP1: A.L. Mahul-Mellier, et al.; Biochim. Biophys. Acta
1823, 1353 (2012),
Abstract;
NF-kappaB regulates thrombin-induced ICAM-1 gene expression in cooperation with NFAT by binding to the intronic NF-kappaB site in the ICAM-1 gene: J. Xue, et al.; Physiol. Genomics
38, 42 (2009),
Abstract;
Full Text
The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed: L. Riol-Blanco, et al.; J. Immunol.
174, 4070 (2005),
Abstract;
Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol.
16, 467 (2004),
Abstract;
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol.
171, 1078 (2003),
Abstract;
Full Text
Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes: O. Micheau & J. Tschopp; Cell
114, 181 (2003),
Abstract;
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem.
278, 18368 (2003),
Abstract;
Full Text
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med.
187, 1205 (1998),
Abstract;
Full Text
Related Products
