Storage and dilution buffer for use with the following products:
KillerTRAIL™, Soluble (human) (recombinant) (Prod. No. ALX-201-073)
SUPERKILLERTRAIL® , Soluble (human) (recombinant) (Prod. No. ALX-201-115)
Useful for prediluting the above mentioned products before preparing aliquots or performing experiments. Predilution with KillerTRAIL™ Storage and Dilution Buffer allows for easier handling of small working concentrations (sometimes only 0.1µl of the KillerTRAIL™ or SUPERKILLERTRAIL® stock solution is necessary because of their high activity). Predilution with KillerTRAIL™ Storage and Dilution Buffer instead of medium or PBS prevents loss of biological activity. Can also be used as negative control.
Product Specification
| Formulation: | 20mM HEPES, pH 7.4, containing 300mM NaCl, 0.01% Tween 20, 1% sucrose and 1mM DTT. |
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| Purity Detail: | Sterile filtered (0.22 µm). |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Technical Info/Product Notes: | Prepare a 1:5 dilution of KillerTRAIL™ or SUPERKILLERTRAIL® with KillerTRAIL™ Storage and Dilution Buffer. For immediate use, keep prediluted KillerTRAIL™ or SUPERKILLERTRAIL® on ice until adding to cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Bortezomib Sensitizes Malignant Human Glioma Cells to TRAIL, Mediated by Inhibition of the NF-κB Signaling Pathway: E.P. Jane, et al.; Mol. Cancer Ther.
10, 198 (2011),
Application(s): Dilution and storage of SuperKillerTRAIL,
Abstract;
Full Text
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