Inhibitor of DNA methyltransferase. Restores mRNA and protein expression of caspase-8 and TRAIL (tumor necrosis factor-related apoptosis inducing ligand) sensitivity of resistant cell lines. Enhances apoptosis induced by HDAC (histone deacetylase) inhibitors. Reverses embryonic stem cell differentiation by demethylating differentiation-related genes.
Product Details
Alternative Name: | 5-Aza-CdR, 5-Aza-dC, 5-Deoxy-2'-azacytidine |
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Formula: | C8H12N4O4 |
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MW: | 228.2 |
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CAS: | 2353-33-5 |
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RTECS: | XZ3012000 |
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Purity: | ≥98% (HPLC) |
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Appearance: | White to off-white lyophilized solid. |
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Solubility: | 25mg/ml soluble in DMSO or 50% acetic acid; 1mg/ml soluble in methanol. (Warming may be required to achieve complete solubilization.) |
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Shipping: | Ambient |
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Long Term Storage: | +4°C |
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Handling: | Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
5-Aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation: S. Takebayashi, et al.; BBRC
288, 921 (2001),
Abstract;
DNA methyltransferase inhibition enhances apoptosis induced by histone deacetylase inhibitors: W.G. Zhu, et al.; Cancer Res.
61, 1327 (2001),
Abstract;
Full Text
Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression: A. Eggert, et al.; Cancer Res.
61, 1314 (2001),
Abstract;
Full Text
Loss of caspase-8 expression in highly malignant human neuroblastoma cells correlates with resistance to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis: S. Hopkins-Donaldson, et al.; Cancer Res.
60, 4315 (2000),
Abstract;
Full Text
The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: implications for methylation-associated cellular processes: T. Haaf; Pharmacol. Ther.
65, 19 (1995),
Abstract;
Cellular differentiation, cytidine analogs and DNA methylation: P.A. Jones and S.M. Taylor; Cell
20, 85 (1980),
Abstract;
Incorporation of a potent antileukemic agent, 5-aza-2'-deoxycytidine, into DNA of cells from leukemic mice: J. Vesely and A. Cihak; Cancer Res.
37, 3684 (1977),
Abstract;