Cell permeable necrosis inhibitor with antioxidant activity. It localizes mostly in the mitochondria. Selectively blocks oxidative stress-induced necrotic cell death (0.1μM NecroX™-5 prevented ~50% cell death in H9C2 cells exposed to 400μM t-BuOOH for 2 hours LDH assay used). Does not protect against staurosporine or etoposide-induced apoptosis. Protects cells against cold shock, hypoxia and oxidative stress in vitro, as well as CCl4 -induced acute liver injury and chronic liver fibrosis in rodent models.
Product Details
| Alternative Name: | [5-(1,1-Dioxo-thiomorpholin-4-ylmethyl)-2-phenyl-1H-indol-7-yl]-(tetrahydro-pyran-4-ylmethyl)-amine |
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| Formula: | C25H31N3O3S . 2 HCl |
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| MW: | 453.6 . 72.9 |
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| Purity: | ≥96% (HPLC, TLC) |
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| Identity: | Determined by LC-MS. |
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| Appearance: | Yellow solid. |
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| Solubility: | Soluble in DMSO (>10mg/ml). |
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| Application Notes: | Can be used for in vitro and in vivo applications.
For in vitro experiments, dissolve Necrox-5™ in DMSO to 20-50mM stock solution and dilute further in cell culture media. Treat cells with 30μM to 0.1μM final concentration.
For in vivo experiments, dissolve the product in DMSO at a concentration of 10mg/ml stock. Do not use PBS and Water!
Optimal conditions must be determined individually for each application. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. Keep under inert gas. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Induction of Lysosomal Membrane Permeabilization Is a Major Event of FTY720-Mediated Non-Apoptotic Cell Death in Human Glioma Cells: K. Min, et al.; Cancers (Basel)
12, 3388 (2020),
Abstract;
Necrotic cell death in atherosclerosis: W. Martinet, et al.; Basic Res. Cardiol.
106, 749 (2011),
Abstract;
NecroX as a novel class of mitochondrial reactive oxygen species and ONOO(-) scavenger: H.J. Kim, et al.; Arch. Pharm. Res.
33, 1813 (2010),
Abstract;
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