Nitrone spin trap for the specific in vivo or in vitro detection of short-lived superoxide, hydroxyl and thiyl radicals. Forms distinguishable adducts which can be measured by EPR spectroscopy. Unlike with DMPO, the superoxide adduct does not decay into a hydroxyl adduct and it has a much longer half-life (t1/2=23min). Low paramagnetic impurities. No further purification required.
Product Specification
| Alternative Name: | 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide |
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| Formula: | C10H17NO3 |
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| MW: | 199.3 |
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| Purity: | ≥99% (HPLC) |
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| Appearance: | White to pinkish crystalline solid. |
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| Solubility: | Soluble in water. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from moisture. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Reactive Oxygen Species Formed by Secondary Organic Aerosols in Water and Surrogate Lung Fluid: H. Tong, et al.; Environ. Sci. Technol.
52, 11651 (2018),
Abstract;
Reactive oxygen species formed in aqueous mixtures of secondary organicaerosols and mineral dust influencing cloud chemistry and public health in the Anthropocene: H. Tong, et al.; Faraday Discuss.
200, 251 (2017),
Application(s): ROS detection in aqueous mixtures of SOA by applying electron paramagnetic resonance (EPR) spectrometry,
Abstract;
Full Text
Oxidation of the Flavonolignan Silybin. In situ EPR Evidence of the Spin-Trapped Silybin Radical: R. Sokolova, et al.; Electrochim. Acta 205, 118 (2016), Application(s): in situ EPR spectroelectrochemistry,
Mechanism of the hydroxyl radical oxidation of methacryloyl peroxynitrate (MPAN) and its pathway toward secondary organic aerosol formation in the atmosphere: T.B. Nguyen, et al.; Phys. Chem. Chem. Phys.
17, 17914 (2015),
Application(s): Trapping agent of OH radicals,
Abstract;
Full Text
S-allyl cysteine alleviates nonsteroidal anti-inflammatory drug-induced gastric mucosal damages by increasing cyclooxygenase-2 inhibition, heme oxygenase-1 induction, and histone deacetylation inhibition: J.M. Park, et al.; J. Gastroenterol. Hepatol.
29 Suppl. 4, 80 (2014),
Application(s): Electron spin resonance (ESR) spectroscopy,
Abstract;
Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence: H. Zhao, et al.; PNAS
102, 5727 (2005),
Abstract;
Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species: H. Shi, et al.; Arch. Biochem. Biophys.
437, 59 (2005),
Abstract;
Spin traps: in vitro toxicity and stability of radical adducts: N. Khan, et al.; Free Radic. Biol. Med.
34, 1473 (2003),
Abstract;
Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals: H. Zhao, et al.; Free Radic. Biol. Med.
31, 599 (2001),
Abstract;
