Nitric oxide (NO) donor.
Product Details
| Alternative Name: | DEA/NO, 2-(N,N-Diethylamino)-diazenolate-2-oxide . diethylammonium salt |
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| Formula: | C4H10N3O2 . C4H12N |
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| MW: | 132.1 . 74.1 |
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| CAS: | 372965-00-9 |
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| Purity: | ≥97% (NMR) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in 100% ethanol (25mg/ml), DMSO (2mg/ml), dimethyl formamide (2mg/ml) or PBS, pH 7.2 (10mg/ml). |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Store, as supplied, at-80°C for up to 1 year. Aqueous solutions should be prepared fresh daily. |
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| Handling: | Protect from light and oxygen. Packaged under inert gas. Hygroscopic. |
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| Technical Info/Product Notes: | Dissociates to the free amine and nitric oxide in a pH-dependent manner following first order kinetics. To initiate the release of nitric oxide, add stock alkaline solution of DEA NONOate to excess buffer of pH 7.0 - 7.4. The half life in 0.1 M phosphate buffer, pH 7.4, is t½ = 16 min. at 22 -25 °C or t½= 2 min. at 37 °C. Liberates 1.5 mol of NO per mol of parent compound. Decomposition is nearly instantaneous at pH 5.0. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The effect of short-term refrigeration on platelet responsiveness: A. Kobsar, et al.; Sci. Rep.
12, 16910 (2022),
Abstract;
Influence of long-term proteasome inhibition on platelet responsiveness mediated by bortezomib: P. Klingler, et al.; Vascul. Pharmacol.
2021, 106830 (2021),
Abstract;
AMPK and Endothelial Nitric Oxide Synthase Signaling Regulates K-Ras Plasma Membrane Interactions via Cyclic GMP-Dependent Protein Kinase 2: K.J. Cho, et al.; Mol. Cell. Biol.
36, 3086 (2016),
Abstract;
Involvement of Heme Oxygenase-1 in particulate matter-induced impairment of NO-dependent relaxation in rat intralobar pulmonary arteries: M. Barrier, et al.; Toxicol. In Vitro
32, 205 (2016),
Application(s): Cell culture,
Abstract;
Endogenous oestrogens do not regulate endothelial nitric oxide production in early postnatal rats: S.I. Sofronova, et al.; Eur. J. Pharmacol.
765, 598 (2015),
Abstract;
Key bioactive reaction products of the NO/H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl: M.M. Cortese-Krott, et al.; PNAS
112, e4651 (2015),
Application(s): Cell culture,
Abstract;
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Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation: M. Shelly, et al.; Science
327, 547 (2010),
Abstract;
Effect of endogenous and exogenous nitric oxide on calcium sparks as targets for vasodilation in rat cerebral artery: M. Mandala, et al.; Nitric Oxide
16, 104 (2007),
Abstract;
Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradients: J.S. Isenberg, et al.; Free Radic. Biol. Med.
40, 1028 (2006),
Abstract;
Nitric oxide mediates interactions between GABAA receptors and adenosine A1 receptors in the rat hippocampus: I.R. Fragata, et al.; Eur. J. Pharmacol.
543, 32 (2006),
Abstract;
A potential role for extracellular nitric oxide generation in cGMP-independent inhibition of human platelet aggregation: biochemical and pharmacological considerations: M.S. Crane, et al.; Br. J. Pharmacol.
144, 849 (2005),
Abstract;
Flavin-containing monooxygenase activity can be inhibited by nitric oxide-mediated S-nitrosylation: S.D. Ryu, et al.; Life Sci.
75, 2559 (2004),
Abstract;
Peroxynitrite-mediated inactivation of heme oxygenases: R. Kinobe, et al.; BMC Pharmacol.
21, 4 (2004),
Abstract;
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New nitric oxide-releasing zwitterions derived from polyamines : J.A. Hrabie, et al.; J. Org. Chem. 58, 1472 (1993),
Complexes of .NO with nucleophiles as agents for the controlled biological release of nitric oxide. Vasorelaxant effects: C.M. Maragos, et al.; J. Med. Chem.
34, 3242 (1991),
Abstract;
DNA deaminating ability and genotoxicity of nitric oxide and its progenitors: D.A. Wink, et al.; Science
254, 1001 (1991),
Abstract;
The Reaction of Nitrogen(II) Oxide with Various Primary and Secondary Amines: R.S. Drago & B.R. Karstetter; JACS 83, 1819 (1961),
The Reaction of Nitrogen(II) Oxide with Diethylamine: R.S. Drago & F.E. Paulik; JACS 82, 96 (1960),
