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Protein synthesis inhibitor
ALX-380-269-G001 1 g 76.00 USD
ALX-380-269-G005 5 g 218.00 USD
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Replaces Prod. #: BML-GR310

Antibiotic. Inhibits protein synthesis in eukaryotes by interfering with the translocation step. Induces apoptosis in tumor cell lines but can also delay or inhibit apoptosis induced by other agents. Widely used for selection of resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression. At 10µg/ml cycloheximide totally inhibits protein synthesis in mouse thymocytes.

Product Details

Alternative Name:Actidione, Naramycin A, 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide
Source:Isolated from Streptomyces griseus.
MI:14: 2728
Purity:≥90% (HPLC)
Appearance:White to light yellow powder.
Activity:Potency is >900units/mg.
Solubility:Soluble in acetone, chloroform, ethanol, and methanol. Nearly insoluble in water.
Shipping:Ambient Temperature - Dangerous Good
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
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Product Literature References

N4BP1 functions as a dimerization-dependent linear ubiquitin reader which regulates TNF signalling: K.W. Kliza, et al.; Cell Death Discov. (2024), Abstract;
Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci. 13, 48 (2022), Abstract;
piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer: S. Wang, et al.; Cell Death Dis. 13, 465 (2022), Abstract;
The Dual Histone Deacetylase-Proteasome Inhibitor RTS-V5 Acts Synergistically With Ritonavir to Induce Endoplasmic Reticulum Stress in Bladder Cancer Cells: K. Okubo, et al.; Anticancer Res. 41, 5987 (2021), Abstract;
The Dual Histone Deacetylase-Proteasome Inhibitor RTS-V5 Acts Synergistically With Ritonavir to Induce Endoplasmic Reticulum Stress in Bladder Cancer Cells: K. Okubo, et al.; Anticancer Res. 41, 5987 (2021), Abstract;
Autophagy is a gatekeeper of hepatic differentiation and carcinogenesis by controlling the degradation of Yap: Y.A. Lee, et al.; Nat. Commun. 9, 4962 (2018), Abstract; Full Text
An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response: A. De Gassart, et al.; PNAS 113, E117 (2016), Application(s): Cell culture, Abstract; Full Text
Bombyx E75 isoforms display stage- and tissue-specific responses to 20-hydroxyecdysone: K. Li, et al.; Sci. Rep. 5, 12114 (2015), Application(s): Cell Culture, Abstract; Full Text
Specific dual effect of cycloheximide on B lymphocyte apoptosis: involvement of CPP32/caspase-3: Cl Lemaire et al.; Biochem. Pharmacol. 58, 85 (1999), Abstract;
Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties: C. Christner, et al.; J. Med. Chem. 42, 3615 (1999), Abstract;
Calpain inhibitors and serine protease inhibitors can produce apoptosis in HL-60 cells: Q. Lu & R.L. Mellgren; Arch. Biochem. Biophys. 334, 175 (1996), Abstract;
A novel protein expressed in mammalian cells undergoing apoptosis: R.J. Grand et al.; Exp. Cell. Res. 218, 439 (1995), Abstract;
Mercuric chloride-induced programmed cell death of a murine T cell hybridoma. I. Effect of the proto-oncogene Bcl-2: J. Alten et al.; Cell. Immunol. 161, 98 (1995), Abstract;
Reevaluation of the role of de novo protein synthesis in rat thymocyte apoptosis: S.C. Chow, et al.; Exp. Cell Res. 216, 149 (1995), Abstract;
Regulation of bcl-2, bcl-XL and bax in the control of apoptosis by hematopoietic cytokines and dexamethasone: J. Lotem & L. Sachs; Cell. Growth. Differ. 6, 647 (1995), Abstract;
DNA fragmentation induced in macrophages by gliotoxin does not require protein synthesis and is preceded by raised inositol triphosphate levels: P. Waring; J. Biol. Chem. 265, 14476 (1990), Abstract; Full Text
Early estrogen-induced metabolic changes and their inhibition by actinomycin D and cycloheximide in human breast cancer cells: 31P and 13C NMR studies: M. Neeman & H. Degani; PNAS 86, 5585 (1989), Abstract; Full Text
Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation in rat fibroblasts: R.B. Widelitz, et al.; Mol. Cell. Biol. 6, 1088 (1986), Abstract; Full Text
The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes: T.G. Obrig, et al.; J. Biol. Chem. 246, 174 (1971), Abstract; Full Text

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