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Apoptosis inducer. Protein kinase inhibitor.
ALX-380-014-C100 100 µg 66.00 USD
ALX-380-014-C250 250 µg 131.00 USD
ALX-380-014-M001 1 mg 328.00 USD
ALX-380-014-M005 5 mg 458.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: BML-EI156

  • Model apoptosis inducer
  • Potent cell-permeable inhibitor of protein kinases
  • Highly cited
Staurosporine is the reference agent for apoptosis induction (1µM in CHO cells). Staurosporine binds to the ATP binding site and inhibits a variety of protein kinases including protein kinase C (PKC), CDK1/cyclin B (IC50~5nM), CDK2/cyclin A (IC50=7nM), CDK4/cyclin D (IC50=3-10µM), CDK5/p25 (IC50=4nM), GSK-3β (IC50=15nM), and Pim-1 kinase (IC50=10nM). Staurosporine does not inhibit PKC-ζ. Staurosporine also inhibits topoisomerase II directly by blocking transfer of phosphodiester bonds from DNA to active site tyrosine. Other than apoptosis and cytotoxicity, some of the biological effects of staurosporine include regulation of eNOS gene expression and relaxation of smooth mucles.

Product Details

Alternative Name:Antibiotic AM-2282
Source:Isolated from Streptomyces staurosporeus.
MI:14: 8802
Purity:≥99% (HPLC)
Appearance:Off-white to green powder.
Solubility:Soluble in DMF (25mg/ml), DMSO (25mg/ml), or ethyl acetate. Only slightly soluble in chloroform and methanol. Insoluble in water.
Shipping:Ambient Temperature
Long Term Storage:+4°C
Use/Stability:Stable for at least 2 years after receipt when stored +4°C.
Handling:Protect from light and moisture. Store under inert gas.
Technical Info/Product Notes:Replacement for ADI-HPK-112
Regulatory Status:RUO - Research Use Only
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Product Literature References

Screening through Lead Optimization of High Affinity, Allosteric Cyclin-Dependent Kinase 2 (CDK2) Inhibitors as Male Contraceptives That Reduce Sperm Counts in Mice: E.B. Faber, et al.; J. Med. Chem. 66, 1928 (2023), Abstract;
Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair: L. Ma, et al.; Bioact. Mater. 19, 626 (2022), Abstract; Full Text
Apoptotic vesicles inherit SOX2 from pluripotent stem cells to accelerate wound healing by energizing mesenchymal stem cells: Y. Qu, et al.; Acta Biomater. 149, 258 (2022), Abstract;
Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts: D.M. Booth, et al.; STAR Protoc. 3, 101119 (2022), Abstract; Full Text
Helicobacter pylori-induced reactive oxygen species direct turnover of CSN-associated STAMBPL1 and augment apoptotic cell death: S. Chaithongyot & M. Naumann; Cell. Mol. Life Sci. 79, 86 (2022), Abstract;
Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles: B. Van den Broek, et al.; Cell. Commun. Signal. 20, 58 (2022), Abstract; Full Text
Protocol for differential centrifugation-based separation and characterization of apoptotic vesicles derived from human mesenchymal stem cells: D. Chen, et al.; STAR Protoc. 3, 101695 (2022), Abstract; Full Text
Staurosporine-induced cleavage of apoptosis-inducing factor in human fibrosarcoma cells is independent of matrix metalloproteinase-2: W. Bassiouni, et al.; Can. J. Physiol. Pharmacol. 100, 184 (2022), Abstract;
Apoptotic Extracellular Vesicles Ameliorate Multiple Myeloma by Restoring Fas-Mediated Apoptosis: J. Wang, et al.; ACS Nano 15, 14360 (2021), Abstract;
Autophagy displays divergent roles during intermittent amino acid starvation and toxic stress-induced senescence in cultured skeletal muscle cells: D. Bloemberg & J. Quadilatero; J. Cell. Physiol. 236, 3099 (2021), Application(s): Mouse skeletal myoblasts (C2C12) treatment, Abstract;
Epigenetic reprogramming of airway macrophages promotes polarization and inflammation in muco-obstructive lung disease: J. Hey, et al.; Nat. Commun. 12, 6520 (2021), Abstract;
Oxidative bursts of single mitochondria mediate retrograde signaling toward the ER: D.M. Booth, et al.; Mol. Cell 81, 3866 (2021), Abstract; Full Text
Chronic irradiation of human cells reduces histone levels and deregulates gene expression: D.J. Lowe, et al.; Sci. Rep. 10, 2200 (2020), Abstract; Full Text
Divergent effects of canonical and non-canonical TGF-β signalling on mixed contractile-synthetic smooth muscle cell phenotype in human Marfan syndrome aortic root aneurysms: A.J. Pedroza, et al.; J. Cell. Mol. Med. 24, 2369 (2020), Abstract; Full Text
Docosahexaenoic acid protection against palmitic acid‐induced lipotoxicity in NGF‐differentiated PC12 cells involves enhancement of autophagy and inhibition of apoptosis and necroptosis: M.L. Montero, et al.; J. Neurochem. 155, 559 (2020), Abstract; Full Text
Gold/alpha-lactalbumin nanoprobes for the imaging and treatment of breast cancer: J. Yang, et al.; Nat. Biomed. Eng. 4, 686 (2020), Abstract; Full Text
Mithramycin selectively attenuates DNA-damage-induced neuronal cell death: O. Makarevich, et al.; Cell Death Dis. 11, 587 (2020), Abstract; Full Text
Resolvin D1 promotes the targeting and clearance of necroptotic cells: B.D. Gerlach, et al.; Cell Death Differ. 27, 525 (2020), Abstract; Full Text
Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge Crambe tailliezi: T.N. Nguyen, et al.; Mar. Drugs 17, 569 (2019), Abstract; Full Text
Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux: D.P. Dannheisig, et al.; Cells 8, 894 (2019), Abstract; Full Text
Membrane Localization of HspA1A, a Stress Inducible 70-kDa Heat-Shock Protein, Depends on Its Interaction with Intracellular Phosphatidylserine: A.D. Bilog, et al.; Biomolecules 9, 152 (2019), Abstract; Full Text
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells: S. Zielke, et al.; Cell Death Dis. 9, 994 (2018), Abstract; Full Text
Cobalamin-Associated Superoxide Scavenging in Neuronal Cells Is a Potential Mechanism for Vitamin B12-Deprivation Optic Neuropathy: W. Chan, et al.; Am. J. Pathol. 188, 160 (2017), Abstract;
Pyroptosis and apoptosis pathways engage in bidirectional crosstalk in monocytes and macrophages: C.Y. Taabazuing, et al.; Cell Chem. Biol. 24, 507 (2017), Abstract; Full Text
The Wnt Target Protein Peter Pan Defines a Novel p53-independent Nucleolar Stress-Response Pathway: A.S. Pfister, et al.; J. Biol. Chem. 290, 10905 (2017), Abstract; Full Text
Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res. 16, 30161 (2016), Application(s): Flow cytometry analysis, Abstract;
Genetically encoded far-red fluorescent sensors for caspase-3 activity: O.A. Zlobovskaya, et al.; Biotechniques. 60, 62 (2016), Application(s): Induced apoptosis, Abstract; Full Text
Recurrent Loss of NFE2L2 Exon 2 Is a Mechanism for Nrf2 Pathway Activation in Human Cancers: L.D. Goldstein, et al.; Cell Rep. 16, 2605 (2016), Application(s): Cell Viability and DNA Fragmentation Analysis, Abstract;
Targeting of nucleotide-binding proteins by HAMLET-a conserved tumor cell death mechanism: J.C. Ho, et al.; Oncogene 35, 897 (2016), Application(s): Cell Culture, Abstract;
Triggering of Suicidal Erythrocyte Death by Bexarotene: A. Al Mamun Bhuyan, et al.; Cell. Physiol. Biochem. 40, 1239 (2016), Abstract;
A small molecule with anticancer and antimetastatic activities induces rapid mitochondrial associated necrosis in breast cancer: A. Bastian, et al.; J. Pharmacol. Exp. Ther. 353, 392 (2015), Application(s): Western Blotting, Abstract; Full Text
Artesunate induces apoptosis via a ROS-independent and Bax-mediated intrinsic pathway in HepG2 cells: G. Qin, et al.; Exp. Cell Res. 336, 308 (2015), Application(s): Cell Culture, Abstract;
Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).: J. Mani, et al.; BMC Cancer 15, 224 (2015), Application(s): Cell Culture, Abstract; Full Text
The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway: A.S. Pfister, et al.; J. Biol. Chem. 290, 10905 (2015), Application(s): Cell Culture, Abstract; Full Text
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions: L. Swain, et al.; J. Leukoc. Biol. 96, 365 (2014), Abstract;
Clostridium difficile Toxin B causes epithelial cell necrosis through an autoprocessing-independent mechanism: N.M. Chumbler, et al.; PLoS Pathog. 8, e1003072 (2012), Abstract; Full Text
Distinct roles of mitochondria- and ER-localized Bcl-xL in apoptosisresistance and Ca2+ homeostasis: C.O. Eno, et al.; Mol. Biol. Cell. 23, 2605 (2012), Abstract; Full Text
Staurosporine and cytochalasin D induce chondrogenesis by regulation of actin dynamics in different way: M. Kim, et al.; Exp. Mol. Med. 44, 521 (2012), Abstract; Full Text
Curcumin attenuates staurosporine-mediated death of retinal ganglion cells: B. Burugula, et al.; Invest. Ophthalmol. Vis. Sci. 52, 4263 (2011), Abstract; Full Text
FKBP51 protects 661w cell culture from staurosporine-induced apoptosis: D.R. Daudt & T. Yorio; Mol. Vis. 17, 1172 (2011), Abstract; Full Text
Neuronal differentiation by analogs of staurosporine: A.F. Thompson & L.A. Levin; Neurochem. Int. 56, 554 (2010), Abstract; Full Text
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Abstract; Full Text
Pim-1 ligand-bound structures reveal the mechanism of serine/threonine kinase inhibition by LY294002: M.D. Jacobs, et al.; J. Biol. Chem. 280, 13728 (2005), Abstract;
Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3: M. Rehm, et al.; J. Biol. Chem. 277, 24506 (2002), Abstract; Full Text
Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a staurosporine-insensitive kinase: M.M. Hill, et al.; J. Biol. Chem. 276, 25643 (2001), Abstract; Full Text
Caspase-8 activation and bid cleavage contribute to MCF7 cellular execution in a caspase-3-dependent manner during staurosporine-mediated apoptosis: D. Tang, et al.; J. Biol. Chem. 275, 9303 (2000), Abstract; Full Text
Changes in mitochondrial membrane potential during staurosporine- induced apoptosis in Jurkat cells: J.L. Scarlett, et al.; FEBS Lett. 475, 267 (2000), Abstract;
Dissociation of staurosporine-induced apoptosis from G2-M arrest in SW620 human colonic carcinoma cells: initiation of the apoptotic cascade is associated with elevation of the mitochondrial membrane potential (deltapsim): B.G. Heerdt, et al.; Cancer Res. 60, 6704 (2000), Abstract;
Equivalent death of P-glycoprotein expressing and nonexpressing cells induced by the protein kinase C inhibitor staurosporine: K.M. Tainton, et al.; BBRC 276, 231 (2000), Abstract;
Glycogen synthase kinase-3beta facilitates staurosporine- and heat shock-induced apoptosis. Protection by lithium: G.N. Bijur, et al.; J. Biol. Chem. 275, 7583 (2000), Abstract; Full Text
Molecular mechanism of staurosporine-induced apoptosis in osteoblasts: H.J. Chae, et al.; Pharmacol. Res. 42, 373 (2000), Abstract;
Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c: D.G. Kirsch, et al.; J. Biol. Chem. 274, 21155 (1999), Abstract; Full Text
Characterization of the cell death process induced by staurosporine in human neuroblastoma cell lines: J. Boix, et al.; Neuropharmacology 36, 811 (1997), Abstract;
Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis: X. Wang et al.; EMBO J. 15, 1012 (1996), Abstract;
K252a and staurosporine microbial alkaloid toxins as prototype of neurotropic drugs: P. Lazarovici, et al.; Adv. Exp. Med. Biol. 391, 367 (1996), Review, Abstract;
Mechanism of topoisomerase II inhibition by staurosporine and other protein kinase inhibitors: P. Lassota et al.; J. Biol. Chem. 271, 26418 (1996), Abstract;
Staurosporine and ent-staurosporine: the first total synthesis, prospects for a regioselective approach, and activity profile: J.T. Link et al.; J. Am. Chem. Soc. 118, 2825 (1996),
Staurosporine induces programmed cell death in embryonic neurons and activation of the ceramide pathway: D.A. Wiesner & G. Dawson; J. Neurochem 66, 1418 (1996), Abstract;
First total synthesis of Staurosporine and ent-Staurosporine: J.T. Link et al.; J. Am. Chem. Soc. 117, 552 (1995),
Differential inhibition of protein kinase C isozymes by UCN-01, a staurosporine analogue: C.M Seynaeve et al.; Mol. Pharmacol. 45, 1207 (1994), Abstract;
Induction of a common pathway of apoptosis by staurosporine: R. Bertrand, et al.; Exp. Cell Res. 211, 314 (1994), Abstract;
Is staurosporine a specific inhibitor of protein kinase C in intact porcine coronary arteries?: M. Kageyama, et al.; J. Pharmacol. Exp. Ther. 259, 1019 (1991), Abstract;
Staurosporine: an effective inhibitor for Ca2+/calmodulin-dependent protein kinase II: N. Yanagihara, et al.; J. Neurochem. 56, 294 (1991), Abstract;
Staurosporine, a protein kinase C inhibitor interferes with proliferation of arterial smooth muscle cells: H. Matsumoto & Y. Sasaki; Biochem. Biophys. Res. Commun. 158, 105 (1989), Abstract;
Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases: U.T. Ruegg & G.M. Burgess; TIPS 10, 218 (1989), (Review), Abstract;
Contrasting actions of staurosporine, a protein kinase C inhibitor, on human neutrophils and primary mouse epidermal cells: T. Sako, et al.; Cancer Res. 48, 4646 (1988), Abstract;
Staurosporine inhibits tyrosine-specific protein kinase activity of Rous sarcoma virus transforming protein p60: N. Nakano, et al.; J. Antibiot. (Tokyo) 40, 706 (1987), Abstract;
Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase: T. Tamaoki, et al.; BBRC 135, 397 (1986), Abstract;
Staurosporine, a potent platelet aggregation inhibitor from a Streptomyces species: S. Oka, et al.; Agric. Biol. Chem. 50, 2723 (1986),
A. Furusaki, et al.; J. C. S. Chem. Commun. 800 (1978),
A new alkaloid AM-2282 of Streptomyces origin. Taxonomy, fermentation, isolation and preliminary characterization: S. Omura, et al.; J. Antibiot. (Tokyo) 30, 275 (1977), Abstract;

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