Gentamycin-class aminoglycoside antibiotic. Cytotoxic to prokaryotic and eukaryotic cells. Induces apoptosis. Widely employed in the selection of eukaryotic expression vectors, in combination with either aminoglycoside phosphotransferase 3’ or APH II.
Product Details
| Alternative Name: | Geneticin |
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| Formula: | C20H40N4O10 . 2H2SO4 |
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| MW: | 496.6 . 196.1 |
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| Source: | Isolated from Micromonospora rhodorangea. |
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| CAS: | 108321-42-2 |
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| RTECS: | CB9378500 |
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| Identity: | Determined by IR. |
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| Appearance: | White to off-white powder. |
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| Activity: | Potency: ≥700 µg/mg |
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| Solubility: | Soluble in water. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Solutions are stable for 3 to 6 months when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | This product is the disulfide salt form of G418 CAS# 49863-47-0. |
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| Technical Info/Product Notes: | The effective killing concentration of this antibiotic will vary as to the cell type, media, growth conditions, and the cells metabolic rate and position in the cell cycle. Effective concentrations have been reported from 100 µg/ml up to 5 mg/ml or greater. When using G418 in a new cell system, a full dose curve is suggested and with each new lot of G418, several points on that curve should be retested, as the potency determined in a standard B. subtilis assay) may not exactly correlate to your system. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Preclinical studies of the triazolo[1,5-a]pyrimidine derivative WS-716 as a highly potent, specific and orally active P-glycoprotein (P-gp) inhibitor: S.Q. Wang, et al.; Acta Pharm. Sin. B
12, 3263 (2022),
Abstract;
Inhibition of gastric H+,K+-ATPase by new dihydropyrazole derivative KYY-008: T. Fujii, et al.; Biochem. Biophys. Res. Commun.
567, 177 (2021),
Abstract;
Overexpression of ABCC1 Confers Drug Resistance to Betulin: X. Chen, et al.; Front. Oncol.
11, 640656 (2021),
Application(s): Cell treatment (carcinoma cell lines: HEK293),
Abstract;
Full Text
Transcriptional suppression of ribosomal DNA with phase separation: S. Ide, et al.; Sci. Adv.
6, 42 (2020),
Abstract;
Full Text
Remodeling the endoplasmic reticulum proteostasis network restores proteostasis of pathogenic GABAA receptors: Y.L. Fu, et al.; PLoS One
13, e0207948 (2018),
Abstract;
Adropin reduces paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions: C. Yang, et al.; Peptides
81, 29 (2016),
Application(s): Cell culture,
Abstract;
Positive regulation of the enzymatic activity of gastric H+,K+-ATPase by sialylation of its β-subunit: T. Fujii, et al.; Biochim. Biophys. Acta
1858, 1228 (2016),
Application(s): Cell culture,
Abstract;
SIRT1 controls cell proliferation by regulating contact inhibition: E.H. Cho, et al.; Biochem. Biophys. Res. Commun.
478, 868 (2016),
Application(s): Transfection and establishment of stable SIRT1-overexpression cell line,
Abstract;
Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus: I.S. Harahap-Carrillo, et al.; Vaccines (Basel)
3, 503 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
The inhibitory effects of anacardic acid on hepatitis C virus life cycle: J. Hundt, et al.; PLoS One
10, e0117514 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
TODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB (Double Strand Break) Repair: I. Gazy, et al.; PLoS One
10, e0134120 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins: N.B. Nillegoda, et al.; Mol. Biol. Cell
21, 2102 (2010),
Abstract;
Full Text
Expression and distribution of HSP27 in response to G418 in different human breast cancer cell lines: L. Qian, et al.; Histochem. Cell Biol.
126, 593 (2006),
Abstract;
Cytochrome c release and endoplasmic reticulum stress are involved in caspase-dependent apoptosis induced by G418: Q.H. Jin, et al.; Cell. Mol. Life Sci.
61, 1816 (2004),
Abstract;
Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI-anchored proteins: M. Kung, et al.; FEBS Lett.
409, 333 (1997),
Abstract;
Phosphatidylinositol phospholipase C is activated allosterically by the aminoglycoside G418. 2-deoxy-2-fluoro-scyllo-inositol-1-O-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase C: J.C. Morris, et al.; J. Biol. Chem.
271, 15468 (1996),
Abstract;
Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation, and preliminary characterization: G.H. Wagman, et al.; Antimicrob. Agents Chemother.
6, 144 (1974),
Abstract;
