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G418 . sulfate

ALX-380-013-M500 500 mg 48.00 USD
ALX-380-013-G001 1 g 63.00 USD
ALX-380-013-G005 5 g 235.00 USD
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  • Used to select neoR-positive cell lines and plasmids
  • Highly cited
G418 is a gentamycin-class aminoglycoside antibiotic with cytotoxic properties in both prokaryotic and eukaryotic cells. In a cell-free assay, G418 inhibits 80S ribosome and protein elongation during translation. In cells, G418 induces apoptosis and is widely employed in the selection of eukaryotic expression vectors containing the neomycin resistance gene neoR, in combination with either APH(2'') or APH(3').

Product Details

Alternative Name:Geneticin
Biological Activity:ED50 Resistant: ≥ 2500µg/ml and ED50 Sensitive: ≤ 400µg/ml.
Formula:C20H40N4O10 . 2H2SO4
MW:496.6 . 196.1
Source:Isolated from Micromonospora rhodorangea.
Identity:Identity determined by HPLC and IR.
Appearance:White to off-white powder.
Activity:Potency: ≥700 µg/mg
Solubility:Soluble in water.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Solutions are stable for 3 to 6 months when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Scientific Background:This product is the disulfide salt form of G418 CAS# 49863-47-0.
Technical Info/Product Notes:The effective killing concentration of this antibiotic will vary as to the cell type, media, growth conditions, and the cells metabolic rate and position in the cell cycle. Effective concentrations have been reported from 100µg/ml up to 5mg/ml or greater. When using G418 in a new cell system, a full dose curve is suggested and with each new lot of G418, several points on that curve should be retested, as the potency determined in a standard B. subtilis assay may not exactly correlate to your system.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci. 12, 48 (2022), Abstract; Full Text
Preclinical studies of the triazolo[1,5-a]pyrimidine derivative WS-716 as a highly potent, specific and orally active P-glycoprotein (P-gp) inhibitor: S.Q. Wang, et al.; Acta Pharm. Sin. B 12, 3263 (2022), Abstract;
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors: A.L. Whittsette, et al.; iScience 25, 104754 (2022), Abstract; Full Text
Inhibition of gastric H+,K+-ATPase by new dihydropyrazole derivative KYY-008: T. Fujii, et al.; Biochem. Biophys. Res. Commun. 567, 177 (2021), Abstract;
Overexpression of ABCC1 Confers Drug Resistance to Betulin: X. Chen, et al.; Front. Oncol. 11, 640656 (2021), Application(s): Cell treatment (carcinoma cell lines: HEK293), Abstract; Full Text
Pharmacokinetic engineering of OX40-blocking anticalin proteins using monomeric plasma half-life extension domains: M. Siegemund, et al.; Front. Pharmacol. 12, 759337 (2021), Abstract; Full Text
Proteostasis regulators restore function of epilepsy-associated GABAA receptors: X. Di, et al.; Cell Chem. Biol. 28, 46 (2021), Abstract; Full Text
Transcriptional suppression of ribosomal DNA with phase separation: S. Ide, et al.; Sci. Adv. 6, 42 (2020), Abstract; Full Text
IP3 receptor isoforms differently regulate ER-mitochondrial contacts and local calcium transfer: A. Bartok, et al.; Nat. Commun. 10, 3726 (2019), Abstract; Full Text
Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II: R. Nagashima, et al.; J. Cell. Biol. 218, 1511 (2019), Abstract; Full Text
A transient rise in free Mg2+ ions released from ATP-Mg hydrolysis contributes to mitotic chromosome condensation: K. Maeshima, et al.; Curr. Biol. 28, 444 (2018), Abstract;
Remodeling the endoplasmic reticulum proteostasis network restores proteostasis of pathogenic GABAA receptors: Y.L. Fu, et al.; PLoS One 13, e0207948 (2018), Abstract;
Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging: T. Nozaki, et al.; Mol. Cell 67, 282 (2017), Abstract;
Adropin reduces paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions: C. Yang, et al.; Peptides 81, 29 (2016), Application(s): Cell culture, Abstract;
Positive regulation of the enzymatic activity of gastric H+,K+-ATPase by sialylation of its β-subunit: T. Fujii, et al.; Biochim. Biophys. Acta 1858, 1228 (2016), Application(s): Cell culture, Abstract;
SIRT1 controls cell proliferation by regulating contact inhibition: E.H. Cho, et al.; Biochem. Biophys. Res. Commun. 478, 868 (2016), Application(s): Transfection and establishment of stable SIRT1-overexpression cell line, Abstract;
A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme: G. Galore-Haskel, et al.; Oncotarget 6, 28999 (2015), Abstract; Full Text
Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus: I.S. Harahap-Carrillo, et al.; Vaccines (Basel) 3, 503 (2015), Application(s): Cell Culture, Abstract; Full Text
The inhibitory effects of anacardic acid on hepatitis C virus life cycle: J. Hundt, et al.; PLoS One 10, e0117514 (2015), Application(s): Cell Culture, Abstract; Full Text
TODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB (Double Strand Break) Repair: I. Gazy, et al.; PLoS One 10, e0134120 (2015), Application(s): Cell Culture, Abstract; Full Text
Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke: K.E. Hawkins, et al.; Mol. Brain 6, 14 (2013), Abstract; Full Text
Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins: N.B. Nillegoda, et al.; Mol. Biol. Cell 21, 2102 (2010), Abstract; Full Text
Expression and distribution of HSP27 in response to G418 in different human breast cancer cell lines: L. Qian, et al.; Histochem. Cell Biol. 126, 593 (2006), Abstract;
Cytochrome c release and endoplasmic reticulum stress are involved in caspase-dependent apoptosis induced by G418: Q.H. Jin, et al.; Cell. Mol. Life Sci. 61, 1816 (2004), Abstract;
Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI-anchored proteins: M. Kung, et al.; FEBS Lett. 409, 333 (1997), Abstract;
Phosphatidylinositol phospholipase C is activated allosterically by the aminoglycoside G418. 2-deoxy-2-fluoro-scyllo-inositol-1-O-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase C: J.C. Morris, et al.; J. Biol. Chem. 271, 15468 (1996), Abstract;
Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation, and preliminary characterization: G.H. Wagman, et al.; Antimicrob. Agents Chemother. 6, 144 (1974), Abstract;

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