A member of the ecdysteroid family. Analog of ecdysone with similar properties to muristerone A. It is a functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Results show that ponasterone A was able to induce expression of β-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity; EC50= 0.07 µM.
Product Details
Alternative Name: | 25-Deoxy-20-hydroxyecdysone, 25-Deoxyecdysterone, 2β,3β,14α,20R,22R-Pentahydroxy-5β-cholest-7-en-6-one |
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Formula: | C27H44O6 |
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MW: | 464.6 |
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Source: | Synthetic. |
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CAS: | 13408-56-5 |
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MI: | 14: 7588 |
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Purity: | ≥95% (HPLC) |
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Identity: | Identity determined by NMR. |
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Appearance: | White to off-white powder. |
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Solubility: | Soluble in acetic acid, 100% ethanol, methanol or DMSO; insoluble in water. |
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Shipping: | Ambient Temperature |
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Long Term Storage: | -20°C |
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Use/Stability: | To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2µmol). Gently mix to dissolve the product (5 to 10 min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at +4°C or at -20°C. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Development of an in vitro Reporter Gene Assay for Screening the Ecdysone Receptor Agonists in Mysid Crustacean: M. Hirano, et al.; Interdisciplinary Studies on Environmental Chemistry
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Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol.
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Abstract;
Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol.
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Abstract;
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Abstract;
The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol.
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Abstract;
Extraction of ponasterone A and ecdysterone from podocarpaceae and related plants: S. Imai et al.; Steroids
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Abstract;