Replaces Prod. #: BML-GR337
Cell permeable steroidal alkaloid. Structurally similar to cyclopamine (Prod. No. BML-GR334). Inhibits the sonic hedgehog (shh) pathway by interacting with smoothened. Can be used to induce abnormal morphogenesis in a number of experimental models. Tomatidine (Prod. No. BML-GR335) is a useful negative control.
Product Details
| Alternative Name: | (3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one, 11-Ketocyclopamine |
| |
| Formula: | C27H39NO3 |
| |
| MW: | 425.6 |
| |
| CAS: | 469-59-0 |
| |
| MI: | 14: 5264 |
| |
| RTECS: | WG9700000 |
| |
| Purity: | ≥98% (TLC) |
| |
| Appearance: | White to off-white solid. |
| |
| Solubility: | Soluble in DMSO (5mg/ml), 100% ethanol (10mg/ml) or dimethyl formamide (6mg/ml). Slight warming may be required. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stock solutions are stable for up to 3 months when stored at -20°C. |
| |
| Handling: | After reconstitution, prepare aliquots and store at -20°C. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
Product Literature References
A defective response to Hedgehog signaling in disorders of cholesterol biosynthesis: M.K. Cooper, et al.; Nat. Genet.
33, 508 (2003),
Abstract;
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium: C.M. Mistretta, et al.; Dev. Biol.
254, 1 (2003),
Abstract;
Identification of a small molecule inhibitor of the hedgehog signaling pathway: effects on basal cell carcinoma-like lesions: J.A. Williams, et al.; PNAS
100, 4616 (2003),
Abstract;
Full Text
Small molecule modulation of Smoothened activity: J.K. Chen, et al.; PNAS
99, 14071 (2002),
Abstract;
Prx1 and Prx2 are upstream regulators of sonic hedgehog and control cell proliferation during mandibular arch morphogenesis: D. ten Berge, et al.; Development
128, 2929 (2001),
Abstract;
Full Text
Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport: J.P. Incardona, et al.; Dev. Biol.
224, 440 (2000),
Abstract;
Teratogen-mediated inhibition of target tissue response to Shh signaling: M.K. Cooper, et al.; Science
280, 1603 (1998),
Abstract;
Related Products
