Heterodimeric C-type lectin. Activates mammalian platelets via binding and clustering of p62/GPVI-receptors under physiological conditions.
Product Details
| MW: | ~84kDa. |
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| Source: | Isolated from Crotalus durissus terrificus snake venom. |
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| CAS: | 37206-04-5 |
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| UniProt ID: | O93426 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Partially purified by multi-step chromatography. |
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| Activity: | <50ng/ml (minimal concentration to induce maximum activation of washed human platelets). |
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| Application Notes: | Can be used for studies on platelet receptors. |
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| Solubility: | Soluble in organic buffer (HEPES). |
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| Reconstitution: | Reconstitute in 10mM HEPES buffer, pH 7.2. (We recommend adding a carrier protein such as BSA to the reconstitution buffer and any subsequent dilution buffers to prevent absorption on plastic surfaces.) |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solutions are stable for 8 hours at +20°C, for 2 days at +4°C or for 1 month when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Protocol: | Assay performed in aggregometer:
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Incubate 485µl platelets suspension (5x108 platelets/ml) for 1-2 minutes at 37°C until base line is stable.
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Add 5µl 200mM CaCl2.
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Add 5µl 200mM MgCl2.
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Wait until baseline is stable.
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Add 5µl collagen solution (0.5mg/ml) for positive control OR add 5µl convulxin (0.1µg/ml in HEPES).
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Measure aggregation at 37°C.
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Ablation of Collagen VI leads to the release of platelets with altered function: V. Abbonante, et al.; Blood Adv.
5, 5150 (2021),
Abstract;
Combined Antiplatelet Therapy Reduces the Proinflammatory Properties of Activated Platelets: A.C.A. Heinzmann, et al.; TH Open
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Abstract;
Feedback Regulation of Syk by Protein Kinase C in Human Platelets: S. Makhoul, et al.; Int. J. Mol. Sci.
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Abstract;
Hypoxia impairs agonist-induced integrin αIIbβ3 activation and platelet aggregation: K. Kiouptsi, et al.; Sci. Rep.
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Full Text
Coated platelets and severe haemophilia A bleeding phenotype: Is there a connection?: K.K. Lastrapes, et al.; Haemophilia
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Abstract;
Establishment of immunoassay for platelet-derived soluble glycoprotein VI, a novel platelet marker: K. Naitoh, et al.; J. Immunol. Methods
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Application(s): Cell Culture, Assay,
Abstract;
Peroxiredoxin II is an antioxidant enzyme that negatively regulates collagen-stimulated platelet function: J. Y. Jang, et al.; J. Biol. Chem.
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Application(s): Cell Culture ,
Abstract;
Full Text
Resveratrol inhibits collagen-induced platelet stimulation through suppressing NADPH oxidase and oxidative inactivation of SH2 domain-containing protein tyrosine phosphatase-2: J.Y. Jang, et al.; Free Radic. Biol. Med.
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Application(s): Cell culture, Flow Cytometry,
Abstract;
Mice Lacking the SLAM Family Member CD84 Display Unaltered Platelet Function in Hemostasis and Thrombosis: S. Hofmann, et al.; PLoS One
9, e11530 (2014),
Application(s): Flow Cytometry,
Abstract;
Full Text
Paxillin is an intrinsic negative regulator of platelet activation in mice: A. Sakata, et al.; Thromb. J.
12, 1 (2014),
Application(s): Cell Culture,
Abstract;
Full Text
Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways: E.C. Josefsson, et al.; Nat. Commun.
5, 3455 (2014),
Application(s): Cell Culture,
Abstract;
Only severe thrombocytopenia results in bleeding and defective thrombus formation in mice: M. Morowski, et al.; Blood
121, 4938 (2013),
Application(s): Flow Cytometry,
Abstract;
Full Text
Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein: J. Liu, et al.; Nat. Med.
17, 206 (2011),
Abstract;
Diminished contact-dependent reinforcement of Syk activation underlies impaired thrombus growth in mice lacking Semaphorin 4D: K.M. Wannemacher, et al.; Blood
116, 5707 (2010),
Abstract;
The human megakaryocytic cell line UT-7/TPO expresses functional platelet agonist signals mediated through GPVI and thromboxane receptor: T. Kawaguchi, et al.; Cell Biol. Int.
34, 943 (2010),
Abstract;
Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role: A.A. Schmaier, et al.; PNAS USA
106, 21167 (2009),
Abstract;
Full Text
Structure of the snake-venom toxin convulxin: T. Batuwangala, et al.; Acta Crystallogr. D. Biol. Crystallogr.
60, 46 (2004),
Abstract;
Convulxin binds to native, human glycoprotein Ib alpha: S. Kanaji, et al.; J. Biol. Chem.
278, 39452 (2003),
Abstract;
Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases: M. Cicmil, et al.; J. Biol. Chem.
275, 27339 (2000),
Abstract;
Convulxin-induced platelet adhesion and aggregation: involvement of glycoproteins VI and IaIIa: M. Jandrot-Perrus, et al.; Platelets
9, 207 (1998),
Abstract;
Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor: J. Polgar, et al.; J. Biol. Chem.
272, 13576 (1997),
Abstract;
Convulxin, a new toxin from the venom of the South American rattlesnake Crotalus durissus terrificus: J. Prado-Franceschi & O.V. Brazil; Toxicon
19, 875 (1981),
Abstract;
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