Inhibitor of CDK1/cyclin B1 and CDK1/cyclin A. Reversibly arrests human cells at the G2/M border of the cell cycle. Allows for effective cell synchronisation in early mitosis. Downregulates expression of the antiapoptotic proteins Bcl-2 and survivin and blocks p53-mediated induction of p21 and MDM2.
Product Details
| Alternative Name: | (5Z)-2-((Thiophen-2-yl)methylamino)-5-((quinolin-6-yl)methylene)thiazol-4(5H)-one |
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| Formula: | C18H13N3OS2 |
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| MW: | 351.4 |
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| CAS: | 872573-93-8 |
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| Purity: | ≥95% (HPLC) |
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| Identity: | Determined by 1H-NMR and MS. |
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| Appearance: | Brown solid. |
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| Solubility: | Soluble in DMSO (50mM). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C in the dark. |
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| Handling: | Protect from light. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Chromosome arm length, and a species-specific determinant, define chromosome arm width: Y. Kakui, et al.; Cell Rep.
41, 111753 (2022),
Abstract;
Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells: H.W. Lau, et al.; Cell Rep.
37, 109808 (2021),
Abstract;
The phosphatase CTDSPL2 is phosphorylated in mitosis and a target for restraining tumor growth and motility in pancreatic cancer: Y. Xiao, et al.; Cancer Lett.
526, 53 (2021),
Abstract;
Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis: F. Farina, et al.; EMBO J.
38, e99843 (2019),
Abstract;
Full Text
Adducin-1 is essential for spindle pole integrity through its interaction with TPX2: W.H. Hsu, et al.; EMBO Rep.
2018, e45607 (2018),
Abstract;
Dominant-Negative Effects of Adult-Onset Huntingtin Mutations Alter the Division of Human Embryonic Stem Cells-Derived Neural Cells: C. Lopes, et al.; PLoS One.
11, e0148680 (2016),
Application(s): Cell culture,
Abstract;
Full Text
MST2 phosphorylation at serine 385 in mitosis inhibits its tumor suppressing activity: X. Chen, et al.; Cell. Signal.
28, 1826 (2016),
Application(s): CDK1 Inhibitor, identification of the corresponding kinase for MST2 phosphorylation,
Abstract;
Phosphorylation of Astrin regulates its Kinetochore Function: H. Chung, et al.; J. Biol. Chem.
291, 17579 (2016),
Application(s): Cell culture for kinase inhibition,
Abstract;
Full Text
An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis: S. Meunier, et al.; Nat. Commun.
6, 7889 (2015),
Application(s): Cell synchronization,
Abstract;
JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis: H. Tuncay, et al.; Nat. Commun.
6, 8128 (2015),
Application(s): Cell culture ,
Abstract;
Full Text
Oncoprotein YAP Regulates the Spindle Checkpoint Activation in a Mitotic Phosphorylation-dependent Manner through Upregulation of BubR1: S. Yang, et al.; J. Biol. Chem.
290, 6191 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Cyclin-dependent kinase 1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML: K. Kojima, et al.; Cancer Sci.
100, 1128 (2009),
Abstract;
Cell cycle synchronization at the G2/M phase border by reversible inhibition of CDK1: L.T. Vassilev; Cell Cycle
5, 2555 (2006),
Abstract;
Full Text
Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1: L.T. Vassilev, et al.; PNAS
103, 10660 (2006),
Abstract;
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