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ALX-270-375-1 1 Vial 83.00 USD
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Derivative from (E)-4-hydroxynonenal (HNE). Can be used to freshly prepare HNE.

Product Specification

Alternative Name:4-HNE-DA
Formulation:Liquid. 6.8mg in 1ml n-hexane. Yields ~5.2mg aldehyde after hydrolysis.
Purity:≥85% (HPLC)
Shipping:Shipped on Dry Ice
Long Term Storage:-20°C
Use/Stability:Unopened vial is stable for at least one year after receipt.
Handling:Keep cool and dry.
Protocol:Preparation and use of (E)-4-Hydroxynonenal (HNE) from the Acetal (HNE-DA):
  • Evaporate the n-hexane under a gentle stream of nitrogen at RT. Continue to work at +4°C.
  • Add 1ml of 1mM HCl (+4°C) and stir for about 45 minutes or close the vial and shake for 30 minutes.
  • The initially cloudy suspension becomes almost clear as saponification proceeds.
  • The resulting aqueous HNE solution has a pH of 3 and contains ~5.2mg of aldehyde.
  • This solution, if kept at +4°C, is stable for at least 24 hours.
  • If neutralized (see Note) this product is stable for 6 hours.
  • We recommend to use the in situ prepared HNE immediately.
Note: HNE decomposes (polymerizes) in basic solution within a few minutes. Never use alkalies to neutralize the pH, but rather appropriately concentrated buffers. During saponification, methanol is generated. If methanol is undesirable, HNE can be extracted in chloroform or dichloromethane. HNE is only sparingly soluble in water.
Regulatory Status:RUO - Research Use Only
(E)-4-Hydroxynonenal-dimethylacetal reaction
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270-375 (E)-4-Hydroxynonenal-dimethylacetal reaction

Product Literature References

Chronic Oxidative Stress Promotes Molecular Changes Associated with Epithelial Mesenchymal Transition, NRF2, and Breast Cancer Stem Cell Phenotype: A. Cipak Gasparovic, et al.; Antioxidants (Basel) 8, 633 (2019), Abstract; Full Text
Oxidative stress is associated with migraine and migraine-related metabolic risk in females: C. Bernecker, et al.; Eur. J. Neurol. 18, 1233 (2011), Abstract;
In vitro model of bone regeneration with bioactive glass and lipid peroxidation: L. Mrakovcic, et al.; Acta Biochim. Pol. 57, 173 (2010), Abstract; Full Text
Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production: stress biomarker or oxidative stress signalling molecule?: L. Foucaud, et al.; Toxicol. In Vitro 24, 1512 (2010), Abstract;
Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes: H. Esterbauer, R.J. Schaur & H. Zollner; Free Radic. Biol. Med. 11, 81 (1991), Abstract;

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