Irreversible inhibitor of serine proteases. Widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB1 receptor activity. Spleen membranes used for CB2 studies do not require this pretreatment, as FAAH activity is absent in these cells. Inhibits also cysteine proteases like papain (reversible by DTT treatment, Prod. No. ALX-280-001), as well as internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor see AEBSF (Prod. No. ALX-270-022).
Product Details
| Alternative Name: | PMSF, Benzylsulfonyl fluoride |
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| Formula: | C7H7FO2S |
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| MW: | 174.2 |
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| CAS: | 329-98-6 |
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| RTECS: | XT8040000 |
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| Purity: | ≥99% (HPLC) |
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| Appearance: | White to off-white needle crystals. |
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| Solubility: | Soluble in 100% ethanol, methanol or isopropanol; insoluble in water. |
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| Shipping: | Ambient |
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| Long Term Storage: | Ambient |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
PUMILIO competes with AUF1 to control DICER1 RNA levels and miRNA processing: S. Rajasekaran, et al.; Nucleic Acids Res.
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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry: L.M.Q. Liao, et al.; J. Vis. Exp. (2021),
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Inhibition of Cyclic Adenosine Monophosphate-Specific Phosphodiesterase by Various Food Plant-Derived Phytotherapeutic Agents: T. Röhrig, et al.; Medicines (Basel)
4, 80 (2017),
Abstract;
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Identification of Novel Mutations in the LRR-Cap Domain of C21orf2 in Japanese Patients With Retinitis Pigmentosa and Cone-Rod Dystrophy: A. Suga, et al.; Invest. Ophthalmol. Vis. Sci.
57, 4255 (2016),
Application(s): Western blot, isolated retina lysates,
Abstract;
Full Text
Development of autoantibodies against muscle-specific FHL1 in severe inflammatory myopathies: I. Albrecht, et al.; J. Clin. Invest.
125, 4612 (2015),
Application(s): Skeletal muscle cell culture,
Abstract;
Full Text
Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist: D.G. Deutsch & S.A. Chin; Biochem. Pharmacol.
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Abstract;
Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers: G.T. James; Anal. Biochem.
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Abstract;
Kinetic analysis of differences in brain acetylcholinesterase from fish or mammalian sources: D.E. Moss & D.E. Fahrney; Biochem. Pharmacol.
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Abstract;
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