Replaces Prod. #: BML-CN268
A slow, tight binding and highly selective inhibitor of inducible nitric oxide synthase (iNOS/NOS II) in vitro and in vivo. Binds tightly to iNOS with a Kd of 7nM. In contrast, inhibition of nNOS and eNOS is weaker and rapidly reversible with Ki values of 2 and 50µM respectively. Inhibits tumor growth in vivo. Prevents circulatory failure but not organ injury induced by endotoxin. Displays protective effects in oxygen-deprived brain slices.
Product Details
| Formula: | C10H15N3 . 2HCl |
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| MW: | 250.17 |
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| Source: | Synthetic. |
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| CAS: | 214358-33-5 |
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| Purity: | ≥95% (TLC) |
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| Identity: | Determined by NMR. |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in water (up to 25mg/ml), methanol or DMSO. |
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| Shipping: | Ambient |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -20°C |
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| Handling: | Hygroscopic. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Differential Effects of D9 Tetrahydrocannabinol (THC)- and Cannabidiol (CBD)-Based Cannabinoid Treatments on Macrophage Immune Function In Vitro and on Gastrointestinal Inflammation in a Murine Model: Z. Yekhtin, et al.; Biomedicines
10, 1793 (2022),
Abstract;
Inflammatory Cell Death, PANoptosis, Mediated by Cytokines in Diverse Cancer Lineages Inhibits Tumor Growth: R.K.S. Malireddi, et al.; Immunohorizons
5, 568 (2021),
Abstract;
The metabolic adaptation evoked by arginine enhances the effect of radiation in brain metastases: R. Marullo, et al.; Sci. Adv.
7, eabg1964 (2021),
Abstract;
GPR30 Attenuates Myocardial Fibrosis in Diabetic Ovariectomized Female Rats: Role of iNOS Signaling: X. Wang, et al.; DNA Cell Biol.
37, 821 (2018),
Abstract;
Chemically Bonding of Amantadine with Gardenamide A Enhances the Neuroprotective Effects against Corticosterone-Induced Insults in PC12 Cells: J. Zhao, et al.; Int. J. Mol. Sci.
16, 22795 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Levosimendan inhibits interleukin-1β-induced apoptosis through activation of Akt and inhibition of inducible nitric oxide synthase in rat cardiac fibroblasts: M. Okada, et al.; Eur. J. Pharmacol.
769, 86 (2015),
Application(s): Cell culture ,
Abstract;
Pretreatment with Relaxin Does Not Restore NO-Mediated Modulation of Calcium Signal in Coronary Endothelial Cells Isolated from Spontaneously Hypertensive Rats: S. Nistri, et al.; Molecules
20, 9524 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Toll-like receptor 4 signaling confers cardiac protection against ischemic injury via inducible nitric oxide synthase- and soluble guanylate cyclase-dependent mechanisms: E. Wang, et al.; Anesthesiology
114, 603 (2010),
Abstract;
Full Text
Effects of 1400W, a potent selective inducible NOS inhibitor, on histamine- and leukotriene D4-induced relaxation of isolated guinea pig nasal mucosa: Y. Chiba, et al.; Nitric Oxide
15, 142 (2006),
Abstract;
1400W, a potent selective inducible NOS inhibitor, improves histopathological outcome following traumatic brain injury in rats: M. Jafarian-Tehrani, et al.; Nitric Oxide
12, 61 (2005),
Abstract;
Addition of nitric oxide donor S-nitroso-N-acetylcysteine to selective iNOS inhibitor 1400W further improves contractile function in reperfused skeletal muscle: J.U. Barker, et al.; Microsurgery
25, 338 (2005),
Abstract;
Increased vasoconstriction to noradrenaline by 1400W, inhibitor of iNOS, in rats with streptozotocin-induced diabetes: X. Cheng & C.C. Pang; Eur. J. Pharmacol.
484, 263 (2004),
Abstract;
Structural basis for the specificity of the nitric-oxide synthase inhibitors W1400 and Nomega-propyl-L-Arg for the inducible and neuronal isoforms: R. Fedorov, et al.; J. Biol. Chem.
278, 45818 (2003),
Abstract;
Full Text
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem.
277, 25823 (2002),
Abstract;
Full Text
Nitric oxide synthases: structure, function and inhibition: W.K. Alderton, et al.; Biochem. J.
357, 593 (2001), Review,
Abstract;
Full Text
The inhibitory potency and selectivity of arginine substrate site nitric-oxide synthase inhibitors is solely determined by their affinity toward the different isoenzymes: R. Boer, et al.; Mol. Pharmacol.
58, 1026 (2000),
Abstract;
Full Text
1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo: E.P. Garvey, et al.; J. Biol. Chem.
272, 4959 (1997),
Abstract;
Full Text
Actions of isoform-selective and non-selective nitric oxide synthase inhibitors on endotoxin-induced vascular leakage in rat colon: F. Laszlo & B.J.R. Whittle; Eur. J. Pharmacol.
334, 99 (1997),
Abstract;
Selective inhibition of inducible nitric oxide synthase inhibits tumor growth in vivo: studies with 1400W, a novel inhibitor: L.L. Thomsen, et al.; Cancer Res.
57, 3300 (1997),
Abstract;
