Substrate for detection of intracellular caspase activation by flow cytometry and fluorescence microscopy after apoptosis induction in intact hematopoietic cell lines.
Product Details
| Alternative Name: | (L-Asp)2 rhodamine 110 (high purity) . TFA salt |
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| Formula: | C28H24N4O9 . CF3COOH |
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| MW: | 560.5 . 114.0 |
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| Purity: | ≥98% |
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| Appearance: | Off-white to orange powder. |
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| Solubility: | Soluble in DMSO. |
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| Reconstitution: | Reconstitute in DMSO followed by the addition of ethanol at a ratio 1:1. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Aliquots in DMSO:ethanol (1:1) are stable for at least 1 month when stored at -20°C and stable for at least 6 months when stored at -80°C. Use high purity reagents only as the dye is instable in water. Discard surplus aqueous solutions. |
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| Handling: | Protect from light. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | D2R can be used to detect caspase activity (predominantly caspase-3) in intact cells by flow cytometry, fluorescence microscopy as well as in cell extracts followed by analysis with a fluorometer (λex: 488nm/λem 550nm). Free (cleaved) Rhodamine 110 emits light both in the FL1 and FL2 channel. Dual staining is possible by employing the FL3 channel for flow cytometry. At least 160 assays can be performed (100µl sample volume (e.g. whole blood sample) and 100µM D2R). Optimal substrate concentrations for other applications range from 25-50µM D2R and have to be determined empirically for the particular cell system. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Fluorogenic substrates as detectors of caspase activity during natural killer cell-induced apoptosis: M. Los, et al.; Methods Mol. Biol.
121, 155 (2000),
Abstract;
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells: H. Hug, et al.; Biochemistry
38, 13906 (1999),
Abstract;
