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Caspase-9 substrate
ALX-260-081-M001 1 mg 65.00 USD
ALX-260-081-M005 5 mg 164.00 USD
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Replaces Prod. #: BML-P443

Chromogenic substrate for caspase-9 and cysteine proteases. Similar to Ac-LEHD-AMC (Prod. No. ALX-260-080) and Ac-LEHD-AFC (Prod. No. ALX-260-116) but cleavage is monitored colorimetrically by absorbance at 405nm.

Product Specification

Alternative Name:Caspase-9 substrate (chromogenic)
Sequence:Ac-Leu-Glu-His-Asp-pNA (pNA = p-Nitroaniline)
Peptide Content:75-95%
Purity:≥99% (HPLC)
Solubility:Soluble in water or  DMSO. Dilute with buffer, pH 7.5.
Long Term Storage:-20°C
Technical Info/Product Notes:λmax of pNA is 400nm.
Protocol:HEPES-Buffer: 100 mM HEPES, pH 7.5; 20% (v/v)
 glycerol; 5 mM DTT, 0.5 mM EDTA.

Substrate: Prepare 1 mM stock solution in DMSO.
- Induce apoptosis and prepare cell lysate or use recombinant caspase.
- Prepare reaction solution:
30 µl of substrate stock solution + 240 µl HEPES-Buffer + 30 µl of cell lysate.
- Incubate for 1 hour at 37 °C.
- Measure with microplate reader at 400nm.
- Suggested controls: Reaction mixture…
…without substrate.
 …with non-apoptotic cell lysate.
 …with apoptotic cell lysate and caspase inhibitor.

Product Literature References

Cardioprotective effects of Prolame and SNAP are related with nitric oxide production and with diminution of caspases and calpain-1 activities in reperfused rat hearts: N.G. Roman-Anguiano, et al.; PeerJ 7, e7348 (2019), Abstract; Full Text
The preclinical analysis of TW-37 as a potential anti-colorectal cancer cell agent: S. Lei, et al.; PLoS One 12, e0184501 (2017), Abstract; Full Text
Early effects of Aβ1-42 oligomers injection in mice: Involvement of PI3K/Akt/GSK3 and MAPK/ERK1/2 pathways: F. Morroni, et al.; Behav. Brain Res. 314, 106 (2016), Application(s): Determination of caspase-9 activation in tissue lysates, Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;

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