| ALX-260-081-M001 | 1 mg | 77.00 USD |  |
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| ALX-260-081-M005 | 5 mg | 200.00 USD | |
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| Do you need bulk/larger quantities? | |||||
| Alternative Name: | Caspase-9 substrate (chromogenic) |
| Sequence: | Ac-Leu-Glu-His-Asp-pNA (pNA = p-Nitroaniline) |
| Formula: | C29H38N8O11 |
| MW: | 674.7 |
| Formulation: | Lyophilized. |
| Peptide Content: | 75-95% |
| Purity: | ≥99% (HPLC) |
| Solubility: | Soluble in water or DMSO. Dilute with buffer, pH 7.5. |
| Shipping: | Ambient Temperature |
| Long Term Storage: | -20°C |
| Technical Info/Product Notes: | λmax of pNA is 400nm. |
| Protocol: | HEPES-Buffer: 100 mM HEPES, pH 7.5; 20% (v/v) glycerol; 5 mM DTT, 0.5 mM EDTA. Substrate: Prepare 1 mM stock solution in DMSO. - Induce apoptosis and prepare cell lysate or use recombinant caspase. - Prepare reaction solution: 30 µl of substrate stock solution + 240 µl HEPES-Buffer + 30 µl of cell lysate. - Incubate for 1 hour at 37 °C. - Measure with microplate reader at 400nm. - Suggested controls: Reaction mixture… …without substrate. …with non-apoptotic cell lysate. …with apoptotic cell lysate and caspase inhibitor. |
| Regulatory Status: | RUO - Research Use Only |
Ac-LEHD-AMC |
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| Caspase-9 substrate | ||||||
| 292633-16-0, ≥98% (HPLC) | Print as PDF | ||||||
| ALX-260-080-M001 | 1 mg | 66.00 USD | |
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| ALX-260-080-M005 | 5 mg | 199.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Ac-LEHD-AFC |
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| Caspase-9 substrate | ||||||
| 210345-03-2, ≥96% (HPLC) | Print as PDF | ||||||
| ALX-260-116-M005 | 5 mg | 256.00 USD | |
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| ALX-260-116-M010 | 10 mg | 445.00 USD | |
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