Replaces Prod. #: BML-P452
Fluorogenic substrate for caspase-1 (Km=4µM), -4 (Km=31µM);and -5 (Km=15µM). This fluorogenic caspase-1 substrate with an optimal tetrapeptide sequence is cleaved 50-fold more efficiently than Ac-YVAD-AMC (Prod. No. ALX-260-024).
Product Details
Alternative Name: | Caspase-1 substrate (fluorogenic), Caspase-5 substrate (fluorogenic), Caspase-4 substrate (fluorogenic) |
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Sequence: | Ac-Trp-Glu-His-Asp-AMC (AMC=7-Amino-4-methylcoumarin) |
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Formula: | C38H40N8O11 |
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MW: | 784.8 |
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Formulation: | Lyophilized. |
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Peptide Content: | 70-90% |
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Purity: | ≥98% (HPLC) |
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Solubility: | Soluble in DMSO (100mM, 75.5mg/ml). Dilute with distilled water or buffer. |
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Shipping: | Ambient |
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Long Term Storage: | -20°C |
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Handling: | Protect from light and moisture. |
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Technical Info/Product Notes: | AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation: D.Y. Cao, et al.; Immunol. Lett.
244, 28 (2022),
Abstract;
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC6: N. Van Opdenbosch, et al.; Cell Rep.
21, 3427 (2017),
Abstract;
Full Text
Design and application of a fluorogenic assay for monitoring inflammatory caspase activity: R. Ranganathan, et al.; Anal. Biochem.
543, 1 (2017),
Abstract;
Synthesis of a small molecule nitrosocysteine inhibitor to reduce the activity of Caspase-1: C.A. Shamblen & C.E. Karver; DePaul Discoveries
5, Article 5 (2016),
Application(s): Caspase-1 inhibition assay,
Full Text
CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes: T. Hieronymus, et al.; Cell Death Differ.
7, 538 (2000),
Abstract;
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ.
6, 362 (1999),
Application(s): Kinetic constants for cleavage of fluorescent substrates,
Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
Full Text
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