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Substrate for caspase-1, -4 and -5
ALX-260-057-M001 1 mg 120.00 USD
ALX-260-057-M005 5 mg 211.00 USD
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Replaces Prod. #: BML-P452

Fluorogenic substrate for caspase-1 (Km=4µM), -4 (Km=31µM);and -5 (Km=15µM). This fluorogenic caspase-1 substrate with an optimal tetrapeptide sequence is cleaved 50-fold more efficiently than Ac-YVAD-AMC (Prod. No. ALX-260-024).

Product Details

Alternative Name:Caspase-1 substrate (fluorogenic), Caspase-5 substrate (fluorogenic), Caspase-4 substrate (fluorogenic)
Sequence:Ac-Trp-Glu-His-Asp-AMC (AMC=7-Amino-4-methylcoumarin)
Peptide Content:70-90%
Purity:≥98% (HPLC)
Solubility:Soluble in DMSO (100mM, 75.5mg/ml). Dilute with distilled water or buffer.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Handling:Protect from light and moisture.
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
Regulatory Status:RUO - Research Use Only

Product Literature References

A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation: D.Y. Cao, et al.; Immunol. Lett. 244, 28 (2022), Abstract;
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC6: N. Van Opdenbosch, et al.; Cell Rep. 21, 3427 (2017), Abstract; Full Text
Design and application of a fluorogenic assay for monitoring inflammatory caspase activity: R. Ranganathan, et al.; Anal. Biochem. 543, 1 (2017), Abstract;
Synthesis of a small molecule nitrosocysteine inhibitor to reduce the activity of Caspase-1: C.A. Shamblen & C.E. Karver; DePaul Discoveries 5, Article 5 (2016), Application(s): Caspase-1 inhibition assay, Full Text
CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes: T. Hieronymus, et al.; Cell Death Differ. 7, 538 (2000), Abstract;
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999), Application(s): Kinetic constants for cleavage of fluorescent substrates, Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract; Full Text

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Caspase-1 substrate
≥98% (HPLC) | Print as PDF
ALX-260-024-M001 1 mg 63.00 USD
ALX-260-024-M005 5 mg 162.00 USD
Do you need bulk/larger quantities?