Replaces Prod. #: BML-P404
The YVAD-CHO portion of this peptide is a highly-specific, potent (Ki=1 nM) and reversible inhibitor of caspase-1 (interleukin-1β converting enzyme, ICE), whose sequence is based on the pre-interleukin-1β Asp-116 cleavage site for caspase-1. The sequence linked N-terminal to YVAD-CHO (residues 1-16) has been shown to enhance cell permeability. The efficacy of the resulting chimeric peptide, for caspase-1 inhibition, is unknown.
Product Details
| Alternative Name: | Caspase-1 inhibitor (aldehyde) |
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| Sequence: | Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Tyr-Val-Ala-Asp-CHO |
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| Formula: | C97H160N20O24 |
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| MW: | 1990.5 |
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| Peptide Content: | 78-98% |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO (0.25mg/ml). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Preincubate cultured cells 1-2 hrs. with 0.1-2.0µM of the peptide, before inducing for apoptosis. Measure indicator(s) of apoptosis at a number of time points, since the effect of the inhibitor may be a delay in the onset of apoptosis, rather than permanent inhibition. It may also be helpful to determine a minimum effective concentration for the apoptosis inducer and use that concentration, when attempting to observe the effects of this peptide. |
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| Handling: | Keep cool and dry. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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